| 摘要: |
| 采用注射外源激素——鲑鱼促性腺激素释放激素类似物(sGnRHa)和人绒毛膜促性腺激素(HCG)的诱导方法, 研究了外源激素对条斑星鲽雄鱼精子质量的影响。结果表明: 外源sGnRHa 和HCG诱导后条斑星鲽精子质量明显提升, 主要表现为精液粘稠度大为降低、精液流动性和液化能力增强、精子激活率和快速激活率显著提高、快速活动时间和精子寿命延长。同时, 血浆性类固醇激素——睾酮(T)和雌二醇(E2)表达水平明显升高, 在96h 达到峰值。比较两种激素的诱导效果, 以sGnRHa对雄鱼精子质量的改善效果为好, 但与HCG无显著差异。本研究表明, 缓释激素诱导可有效提升和改进条斑星鲽雄鱼的精子质量, 研究结果对条斑星鲽人工繁育具有重要的指导意义。 |
| 关键词: 条斑星鲽, 外源激素诱导, 精子质量 |
| DOI：10.11693/hyhz201206020020 |
| 分类号: |
| 基金项目:国家鲆鲽类现代产业技术体系项目, CARS-50 号; 国家863计划项目, 2012AA10A413 号; 中央级公益性事业单位基本科研业务费项目, 20603022012022 号; 国家留学人员科技活动项目择优资助经费资助, 2011—2013 |
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| EFFECTS OF EXOGENOUS HORMONES INDUCTION ON THE SPERM QUALITY OF BARFIN FLOUNDER VERASPER MOSERI |
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XU Yong-Jiang1,2, LIU Xue-Zhou1,2, WANG Yan-Yan1,2, LIU Xin-Fu1,2
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1.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Science;2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao Ke
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| Abstract: |
| The effects on exogenous hormones including human chorionic gonadotropin (HCG) and salmon gonadotropin releasing hormone analogue (sGnRHa) on sperm quality of male barfin flounder Verasper moseri were investigated in the present study. Results showed that exogenous hormone induction can greatly improve the sperm quality of males with milt of high viscosity, including increased milt fluidity, higher sperm motility and longer sperm life span, and these were associated with elevated serum sex steroids level. The increased expression level of sex steroid peaked at 9h post hormonal induction for both groups, which was in accordance with the sperm quality elevation. The sGnRHa induction beard better effect than that of HCG induction although there was no significant difference observed between sGnRHa and HCG induction. Results from this study can serve as useful tools in acquiring milt with high quality and could be beneficial for artificial insemination in barfin flounder culture. |
| Key words: Barfin flounder Verasper moseri, Exogenous hormones induction, Sperm quality |
