| 摘要: |
| 从浙江台州近海上层海水中分离到一株可产Macrolactin类大环内酯抗生素的海洋细菌, 经形态特征分析及16S rRNA 测序比对, 鉴定并将其命名为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)ESB-2。运用Minitab软件, 通过Plackett-Burman和Box-Behnken实验及响应面分析对该菌生产Macrolactin B的发酵条件进行优化, 得到该菌生产Macrolactin B的最佳发酵条件为: 葡萄糖17.0mg/L, 蛋白胨5mg/L, 酵母粉1mg/L, FePO40.01mg/L, 初始pH 6.0, 培养温度27.2℃, 接种量5%, 装液量57.6%, 培养时间4d, 在优化发酵条件下, Macrolactin B 的最高产量达到13.6?g/ mL, 为目前已报道的最高产量, 为该活性物质药源问题的解决打下了良好基础。 |
| 关键词: 海洋解淀粉芽孢杆菌 Macrolactin B 发酵优化 响应面法 |
| DOI：10.11693/hyhz20120914001 |
| 分类号: |
| 基金项目:浙江省海洋生物技术产业科技创新团队资助项目, 2012R10029-2 号。 |
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| OPTIMIZATION OF CULTURE CONDITIONS FOR PRODUCTION OF MACROLACTIN B BY A MARINE BACILLUS AMYLOLIQUEFACIENS |
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WANG Hong-Qiang, HE Shan, YANG Rui, ZHU Peng, YAN Xiao-Jun
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Ningbo University, Key Laboratory of Applied Marine Biotechnology of Ministry of Education
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| Abstract: |
| A macrolactins-producing marine bacteria were isolated from seawater in Taizhou, Zhejiang Province. This strain was identified and named as Bacillus amyloliquefaciensESB-2 based on phenotypic characteristics and 16S rRNA gene sequencing. Minitab statistical software was applied to optimize the fermentation conditions for the production of macrolactin B fermented by ESB-2 strain in Plackett-Burman and Box-Behnken design. The optimal fermentation conditions for maximum macrolactin B production were Peptone 5mg/mL, yeast extract 1mg/mL, glucose 17.0mg/L, FePO4 0.01mg/mL, temperature 27.2℃, initial pH value 6.0, medium volume 57.6%, rotation speed 150r/min, inoculation 5% and fermented for 4 days. Under the optimization conditions, the concentration of macrolactin B was increased eight-folds and reached 13.6?g/mL. |
| Key words: Bacillus amyloliquefaciens Macrolactin B optimization response surfaces |
