| 摘要: |
| 采用细胞超声破碎-丙酮沉淀及除盐-50mmol/L Tris-HCl (pH 6.8)复溶的方法分别提取光棘球海胆(Strongylocentrotus nudus)雄性、雌性性腺的可溶性全蛋白, 利用载体两性电解质等电聚焦双向电泳分别在pH 5—8和pH 4—6范围内对获得的性腺组织可溶性全蛋白进行分离。通过PDQuest8.0图像分析软件比较分析, 发现pH 5—8范围内, 雄性光棘球海胆性腺双向电泳图谱检出的蛋白质斑点为321个, 雌性光棘球海胆性腺检出蛋白质斑点402个。在pH 4—6范围内, 雄性光棘球海胆性腺双向电泳图谱检出的蛋白质斑点数为239个, 雌性光棘球海胆性腺检出197个蛋白质斑点, 其中, 表达呈现差异性的蛋白质斑点数为208个。结果提示, 不同性别光棘球海胆的性腺蛋白质表达存在显著差异。 |
| 关键词: 性别 光棘球海胆 双向电泳 蛋白质组 性腺 |
| DOI：10.11693/hyhz20120920001 |
| 分类号: |
| 基金项目:大连海洋大学人才引进启动基金, SYYJ2010002 号; 国家自然科学基金“海洋酸化对海胆浮游幼体钙化生长的影响及其机制”, 41206128 号; 国家“863”计划“高值海珍品良种培育”, 2012AA10A412 号; 辽宁省科技计划资助项目, 2007203004 号; 辽宁省“百千万人才工程”资助项目, 2008921059 号; 辽宁省“高等学校杰出青年学者成长计划”资助项目, LJQ2013079 号。 |
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| ANALYSIS OF GONAD PROTEOMES OF DIFFERENT GENDER SEA URCHIN STRONGYLOCENTROTUS NUDUSBY TWO-DIMENSIONAL ELECTROPHORESIS |
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ZHAN Yao-Yao1,2, HUANG Xian-Ya1,2, SHEN Yan1,2, LIU Wei1,2, MU Lin-Lin1, HAO Zhen-Lin1,2, WANG Yi-Nan1,2, DING Jun1,2, CHANG Ya-Qing1,2
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1.College of Fisheries and Life Science, Dalian Ocean University;2.Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture
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| Abstract: |
| We extracted total proteins in male and female gonads of sea urchin Strongylocentrotus nudusby the method of ultrasonication-acetone sedimentate-50mmol/L Tris-HCl (pH 6.8) and then separated by the carrier ampholytes two-dimensional electrophoresis in pH gradient. Results show that: (1) in pH 5-8, proteins in male and female gonads contained 321 and 402 protein spots, respectively; (2) in pH 4—6, they were 239 and 197 protein spots, respectively. Two hundred and eight unmatched protein spots were found in male and female gonad in pH 4—6 by PDQuest8.0 image analysis software, which suggests that there is a significant difference in protein expression level between male and female gonad of S. nudus. |
| Key words: gender Strongylocentrotus nudus two-dimensional electrophoresis proteome gonad |
