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扇贝(Chlamys farreri)多肽通过EGFR抑制受紫外线B(UVB)诱导的HaCaT细胞凋亡
樊洁,韩彦弢,宋秀萍,王春波
山东省精神卫生中心,青岛大学,青岛大学,青岛大学
摘要:
利用Hoechst 33258荧光染色法检测紫外线B(UVB)辐射诱导HaCaT细胞凋亡率。结果表明, 扇贝多肽(Polypeptide from Chlamys farreri, PCF)可以剂量依赖性抑制UVB诱导的HaCaT细胞凋亡; 表皮生长因子受体(EGFR)抑制剂AG1478能明显抑制UVB诱导的HaCaT细胞凋亡。采用3’-RACE法构建EGFR的cDNA片段, 克隆测序检测突变位点。结果表明, UVB 照射后EGFR发生碱基突变A→G,A→G,T→C,G→A,G→A; 预加入5.69mmol/L的PCF, 产生部分抗突变作用, 1, 2, 3突变位点处未发生碱基的突变。
关键词:  扇贝多肽(Polypeptide from Chlamys farreri, PCF)  紫外线B(UVB)  表皮生长因子受体(EGFR)  基因突变  细胞凋亡
DOI：10.11693/hyhz20121125001
分类号:
基金项目:国家自然科学基金资助项目, 30471458 号; 山东省自然科学基金资助项目, Z2007c09 号
POLYPEPTIDE FROM CHLAMYS FARRERICAN INHIBIT THE UVB-INDUCED HACAT CELL APOPTOSIS
FAN Jie,HAN Yan-Tao,SONG Xiu-Ping and WANG Chun-bo
Shandong Mental Health Center,Qingdao University,Qingdao University,Qingdao University
Abstract:
The apoptosis of UVB-exposed HaCaT cells with Hoechest 33258 fluorescent staining was investigated. Result shows that polypeptide from Chlamys farreri(PCF) and AG1478, an inhibitor of epidermal growth factor receptor (EGFR), can inhibit the rate of apoptosis. Using 3’-RACE method, cDNA fragment of EGFR was constructed, and mutation detected by cloning and sequencing. In addition, UVB induced the gene mutation of EGFR (A→G, A→G, T→C, G→A, G→A), and PCF at 5.69 mmol/L could protect EGFR from mutation.
Key words:  polypeptide from Chlamys farreri  ulroviolet B  epidermal growth factor receptor (EGFR)  gene mutation  apoptosis
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