| 摘要: |
| 采用两步降温法超低温冷冻保存大黄鱼精子, 并用透射电镜技术研究了精子的超微结构损伤。结果表明, 大黄鱼冻精的激活率、运动时间及寿命与鲜精相比无显著差异。鲜精中28.5%的精子形态结构异常, 冻精中37%的精子形态结构异常。形态结构正常的精子表现为质膜与核膜结构完整、无膨胀现象, 袖套、轴丝及中心粒结构正常, 线粒体形态完整、嵴较发达; 形态结构异常的精子表现为质膜破裂、脱落, 质膜膨胀, 核膜破裂、脱落, 核局部受损伤, 线粒体膨胀、嵴退化或消失, 线粒体移位或脱落。结果显示, 以Cortland溶液为稀释液, 10% DMSO为抗冻剂, 对大黄鱼精子具有较好的抗冻保护作用。 |
| 关键词: 大黄鱼, 精子, 超低温冻存, 活力, 超微结构 |
| DOI：10.11693/hyhz201301009009 |
| 分类号: |
| 基金项目:国家星火计划项目, 2011GA701001 号; 浙江省水产新品种选育专项, 2012C12907-8 号; 宁波市科技计划重大项目, 2011C11005号; 宁波市科技创新团队项目, 2011B82018 号; 宁波大学优秀学位论文培育基金，PY2012016号; 舟山市科技计划项目, 10206 号 |
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| VITALITY AND ULTRASTRUCTURE OBSERVATION OF FRESH AND CRYOPRESERVATED SPERM IN PSEUDOSCIAENA CROCEA |
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CHENG Shun1, YAN Jia-Qiang1, ZHU Jun-Quan1, JIANG Jian-Hu1, WU Xiong-Fei2, SHI Hui-Lai3
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1.Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, Ningbo University;2.Ningbo Academy of Oceanology and Fisheries;3.Marine Fishery Institute of Zhejiang Province
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| Abstract: |
| A two-step cooling procedure was employed to cryopreserve Pseudosciaena crocea sperm, and the sperm ultrastructure after which was observed under transmission electron microscopy. The results show that there were no significant differences between frozen-thawed sperm and fresh sperm by comparing the activation rate, moving time and life-span. Both the fresh sperm and cryopreserved sperm had ultrastructural damages in various degree. The deformation rate of the fresh and cryopreservated sperms were 28.5% and 37%, respectively. The following aspects of cryopreserved sperm with normal morphology was observed: the plasma and nuclear membrane; the sleeve, axoneme and centriole; the mitochondrion obtained integrity and with well-developed cristae. On the contrary, the sperm cryodamages were observed as follows: swelled or disrupted plasma and nuclear membrane; partially damaged nucleus; the swelled, dislocated or disarticulated mitochondrion with degenerated or vanished cristae. The results show that Cortland solutions and 10% DMSO are the best choice for extender and cryoprotecant, which are helpful for improving the frozen-thawed P. crocea sperm quality. |
| Key words: Pseudosciaena crocea, Sperm, Cryopreservation, Vitality, Ultrastructure |
