| 摘要: |
| 通过设置5个N/P浓度梯度和3个N浓度下的4个N∶P梯度, 进行了江蓠对N、P的短期(0—4h)和长期(第14天)吸收速率(SNUR和LNUR)以及短期和长期吸收效率(SNUE和LNUE)的研究, 同时观察了5个N/P浓度梯度对江蓠细胞超微结构的影响。结果表明: N∶P比为16∶1时, 江蓠对N、P的SNUR和LNUR均最大(P<0.05); N/P为1200/75和960/60μmol/L浓度时, 分别对N和P的SNUR最大, 达12.85和0.79μmol/(gDW·h), 但此N/P浓度下长期培养的江蓠叶绿体结构存在不同程度的损伤; 而N/P浓度480/30μmol/L时, 对N和P的LNUR均最大值4.23和0.31μmol/(gDW·h)。江蓠对N、P的SNUE和LNUE在介质N/P 60/3.75μmol/L时为最高, 而在1200/75μmol/L时为最低(P<0.05)。 |
| 关键词: 江蓠, 氮, 磷, 吸收效应, 超微结构 |
| DOI：10.11693/hyhz201301015015 |
| 分类号: |
| 基金项目:江苏省社会发展基金资助项目, BS2002016 号 |
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| UPTAKE EFFECTS OF NITROGEN AND PHOSPHORUS AND CELL ULTRASTRUCTURE CHANGES OF GRACILARIA LICHENOIDES |
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HUANG He-Zhong1,2, LIANG Jian-Sheng1, ZHANG Qun-Ying2
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1.College of Bioscience and Biotechnology, Yangzhou University;2.School of Basic Medicine and Biological Science, Soochow University
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| Abstract: |
| The effects of variable 5 N/P concentration levels and 4 N∶P ratio levels of 3 N concentration levels, respectively, on uptake rates (SNUR and LNUR) and uptake efficiency (SNUE and LNUE) of N (TIN) and P (PO3-4-P) by Gracilaria lichenoides for short-term (0—4h) and long-term (over a 24h period at 14th day) were studied. Meanwhile, ultrastructure of cells of G. lichenoides cultivated in different N and P concentration for 14 day was observed. The results showed that optimal N∶P ratio in culture media which SNUR and LNUR for N and P by G. lichenoides reached the highest value among different N/P concentrations (P<0.05) was 16∶1. The reached maximum SNUR of N and P by G. lichenoides was respectively 12.85 and 0.79μmol/(gDW·h), when the N/P concentration in medium was 1200/75 and 960/60μmol/L, but the ultrastructure of chloroplasts of G. lichenoides cultivated in those N/P concentration for 14 day emerged some extent damage. The reached maximum LNUR of N and P by G. lichenoides were 4.23 and 0.31μmol/(gDW·h), respectively, when the N/P concentration in medium was 480/30μmol/L. The reached maximum SNUE and LNUE of N and P by G. lichenoides was in the medium of N/P 60/3.75μmol/L, but decreased to the minimum SNUE and LNUE, respectively, was in the medium of N/P 1200/75μmol/L. |
| Key words: Gracilaria lichenoides, Nitrogen, Phosphorus, Uptake effect, Ultrastructure |
