| 摘要: |
| 无乳链球菌是一种人畜鱼共患的重要病原菌, 菌体表面的荚膜多糖是公认的毒力因子, 其合成过程受荚膜多糖合成基因的调控。荚膜多糖合成基因存在于荚膜多糖操纵子中, 参与无乳链球菌荚膜多糖的合成启动、寡糖和多糖的聚合以及外输并锚定于菌体表面, 在新型诊断技术和减毒突变株的构建方面取得良好的应用。本文首次就cps基因的基本属性、转录调节、编码蛋白及其生物功能、对荚膜多糖合成的调控机理、在血清分型和突变株构建的应用这六个方面进行深入分析和讨论, 以期为GBS cps基因的新功能研究和创新应用提供理论参考。 |
| 关键词: 无乳链球菌, 荚膜多糖合成基因, 作用机制, 应用 |
| DOI：10.11693/hyhz201301028028 |
| 分类号: |
| 基金项目:教育部“长江学者和创新团队发展计划”创新团队项目, IRT0848 号; 通威股份有限公司重点资助项目, 2006—2009 |
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| A REVIEW ON THE CAPSULAR POLYSACCHARIDE SYNTHESIS GENES OF STREPTOCOCCUS AGALACTIAE |
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WANG Kai-Yu1,2, HUANG Jin-Lu1, XIAO Dan3, WANG Jun1, HUANG Ling-Yuan1
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1.Fish Disease Research Center of Sichuan Agricultural University;2.Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University;3.Tongwei Co., Ltd.
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| Abstract: |
| Streptococcus agalactiae is an important zoonotic pathogen in human, animal and fish. The capsular polysaccharides on the cell surface have been recognized as virulence factors, of which the forming processes are regulated by the capsular polysaccharide biosynthesis (cps) genes. The capsular polysaccharide biosynthesis genes exist in capsular polysaccharide operon of S. agalactiae genome, which were predicted to participate in the origination of capsular polysaccharide synthesis, polymerization of oligosaccharides and polysaccharides, transport and anchor the product to the cell surface. Therefore, the cpsgenes achieve new applications of diagnostic techniques and the construction of attenuated mutant strains. In this paper, the basic properties of cps genes, as well as their transcription regulation, coding proteins and biological functions, regulation mechanism of capsular polysaccharide biosynthesis and serotyping, and application of construction of mutant strains are analyzed and discussed. We looked forward to providing a theoretical reference on the new functions research and innovative applications of S. agalactiae cps genes. |
| Key words: Streptococcus agalactiae, cps genes, Regulating mechanism, Application |
