| 摘要: |
| 采用膜片钳技术解析了中华绒螯蟹血淋巴细胞表达的大电导钙激活钾通道(BKCa), 研究了中华绒螯蟹BKCa对血淋巴细胞吞噬和杀菌作用的影响, 并探讨了其机制。结果表明, 当Ca2+浓度在0.3—3.0μmol/L范围时, 中华绒螯蟹血淋巴细胞表达的钙激活钾通道的开放表现出明显的钙依赖活性; 培养液中游离Ca2+的添加显著增强了血淋巴细胞的杀菌能力和呼吸爆发活性, 但对其吞噬率和吞噬指数的影响不显著; 在6mmol/L[Ca2+]浓度条件下, 加入不同浓度的KCa 阻断剂四乙胺(Tetraethylammonium, TEA)后, 血淋巴细胞的杀菌能力和呼吸爆发活性均显著降低, 但其吞噬率和吞噬指数并未受到影响。这提示钙激活钾通道可能通过间接调节细胞内的钙离子浓度而在血淋巴细胞杀菌过程中发挥重要作用。 |
| 关键词: 中华绒螯蟹, 血淋巴细胞, 钙激活钾通道, 吞噬, 杀菌, 呼吸爆发 |
| DOI：10.11693/hyhz201301032032 |
| 分类号: |
| 基金项目:国家高技术发展计划项目, 2012AA10A401 号; 天津市应用基础研究计划项目, 10JCZDJC18200 号; 国家科技支撑计划项目, 2012BAD13B07 号; 农业部淡水水产种质资源重点开放实验室课题资助 |
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| EFFECTS OF CALCIUM-ACTIVATED POTASSIUM CHANNELS ON IMMUNE ACTIVITIES OF HAEMOCYTES IN CHINESE MITTEN CRAB ERIOCHEIR SINENSIS |
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ZHANG Yi-Chen1, GAO Xue-Wei2, SUN Yan2, GENG Xu-Yun2, LIU Yi-Chen1, SUN Jin-Sheng1,2
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1.Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Science, Tianjin Normal University;2.Tianjin Aquatic Animal InfectiousDisease Control and Prevention Center
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| Abstract: |
| Inside-out patch clamp technique was used to study the calcium sensitivity of calcium-activated potassium channel (BKCa) on haemocytes from Chinese mitten crab (Eriocheir sinensis). Effects and mechanism of BKCa on phagocytosis and bactericidal activity of haemocytes were also analyzed. The results showed that the opening probability of BKCa channel had significant calcium-dependent activity in the concentration of Ca2+ at the range of 0.3—3.0μmol/L. Though there were no significant effects on the phagocytosis of haemocytes with Ca2+ or TEA adding (P>0.05), the bactericidal and respiratory burst activities were enhanced with appropriate Ca2+ (activator of KCa channel) adding and decreased significantly with 6mmol/L [Ca2+] and different concentration of TEA (inhibitor of KCa channel) adding (P<0.01). It could be deduced that KCachannels play an essential role in innate immunity of crustacean, by regulating the intracellular [Ca2+] indirectly. |
| Key words: Eriocheir sinensis, Haemocytes, Calcium-activated potassium channel, Phagocytosis, Bactericidal
activity, Respiratory burst |
