| 摘要: |
| 采用群落水平和细胞水平碱性磷酸酶活性(APA)相结合的方法, 研究了2010 年夏季(6—7月)九龙江河-海系统(河流-河口-邻近海域)浮游植物的磷胁迫, 结果表明, 单位叶绿素a的APA在河流下游区[(48.80±14.69)nmol/(h·μg Chl a)]显著高于河口区[(10.60±15.58)nmol/(h·μg Chl.a)]和邻近海域[(23.54±25.41)nmol/(h·μg Chl.a)], 表明河流区的浮游植物磷胁迫显著高于河口区和邻近海域。三个区域高比例的游离态APA表明该水域浮游植物已较长时间处于磷胁迫状态。细胞水平的浮游植物荧光标记结果显示, 河口区(20%±20%)与邻近海域(38%±20%)无显著差异(P>0.05), 此结果与群落水平APA结果相一致; 但河口-邻近海域浮游植物的标记比例显著高于河流下游区(9%±3%)(P<0.05)。 |
| 关键词: 浮游植物, 碱性磷酸酶活性(APA), 磷胁迫, 九龙江河-海系统 |
| DOI：10.11693/hyhz201301036036 |
| 分类号: |
| 基金项目:国家自然科学基金项目, 40925018 号, 41176112 号 |
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| PHYTOPLANKTON PHOSPHORUS STRESS OF JIULONGJIANG RIVER-ESTUARY AND ADJACENT WATERS SYSTEM IN SUMMER |
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MO Yu, LIN Li-Zhen, ZHENG Li-Ping, HUANG Bang-Qin
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Fujian Provincial Key Laboratory of Coastal Ecology and Environmental Studies, College of the Environment and Ecology, Xiamen University
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| Abstract: |
| Phosphorus stress of phytoplankton was determined by using bulk and single-cell alkaline phosphatase activity (APA) assays during summer (June—July 2010) in Jiulongjiang River-Estuary and adjacent waters system. Results showed that Chl.a-normalized APA was significantly higher in the lower Jiulongjiang River [(48.80±14.69)nmol/(h·μgChl.a)] than the estuary and adjacent coastal waters [(10.60±15.58)nmol/(h·μgChl.a) and (23.54±25.41)nmol/(h·μgChl.a), respectively], indicating that phytoplankton phosphorus stress was more serious in the lower Jiulongjiang River than that in the estuary and coastal waters. High soluble APA contribution indicated that phytoplankton suffered long-term phosphorus (P) stress in the river-estuary-coastal water system. Single-cell ELF labeling of phytoplankton showed that there was no significant difference between the estuary (20%±20%) and adjacent coastal waters (38%±20%), which was coincided with the results of bulk APA. However ELF labeling of phytoplankton in estuary-coastal waters was significantly higher than that in the lower Jiulongjiang River (9%±3%) (P<0.05). |
| Key words: Phytoplankton, Alkaline phosphatase activity(APA), Phosphorus stress, Jiulongjiang River-Estuary and adjacent waters system |
