| 摘要: |
| 采用透射电镜技术, 进行了哲罗鱼卵巢滤泡细胞发育的研究。结果表明, 哲罗鱼滤泡细胞发育分为5个时期: 零散滤泡膜细胞期、单层扁平滤泡膜细胞期、多层扁平滤泡膜细胞期、颗粒细胞分泌期和颗粒细胞退化期。滤泡细胞经卵巢基质细胞分化, 由卵巢边缘经“穿梭包围运动”将卵母细胞包裹。早期滤泡细胞在卵母细胞外侧呈连续单层排列, 并不具备任何功能, 随着卵母细胞发育, 滤泡细胞分化成具有功能性的三层结构, 随后滤泡细胞协同卵母细胞合成部分卵膜。当卵黄积累旺盛时, 滤泡细胞参与了外源性卵黄物质的运输、加工和修饰, 鞘细胞间大量的微丝具有协助卵巢排卵的作用。在卵巢排卵后, 滤泡细胞与未发育的卵母细胞形成闭锁卵泡, 对卵细胞残体和碎片还具有吞噬、消化等作用。 |
| 关键词: 哲罗鱼, 卵巢, 滤泡细胞, 超微结构 |
| DOI：10.11693/hyhz201301039039 |
| 分类号: |
| 基金项目:国家“十二五”科技支撑计划, 2012BAD25B10 号；农业部公益性行业科研专项, 201003055 号 |
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| STUDY ON DEVELOPMENTAL ULTRASTRUCTURE OF FOLLICULAR CELLS IN OVARY OF HUCHO TAIMEN |
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ZHANG Yong-Quan, YIN Jia-Sheng, DU Jia, ZHANG Ying, TONG Guang-Xiang
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Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences
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| Abstract: |
| The development of follicular cells in ovary of Hucho taimen was studied by transmission electron microscope. The results showed that the development process of follicular cell could be divided into five stages: scattered follicle membranate cells stage, monolayer applanate follicle membranate cells stage, multi-layers applanate follicle membranate cells stage, granulosa cell secretion stage and granulosa cell degeneration stage. Follicular cell that was differentiated from stroma cell of ovary wrapped oocyte in shuttle and envelope of motion from edge of ovary. The outside of oocyte was lined with continuous monolayer pristine follicular cells, which didn’t have any function, with the development of oocyte, follicular cells differentiated to be obvious three-layered structure, whereafter, follicular cells synthesized part of egg membrane in coordination with oocyte. When yolk amassed vigorously, follicular cell participated in transportation, processing and modified of exogenous yolk material, the considerable microfilament of sheath cell assisted ovulation of ovary. After ovulation, follicular cell formed atretic follicle with nulli-developmental oocyte to phagocytize and digeste residuum and fragment of ovotid. |
| Key words: Hucho taimen, Ovary, Follicular cell, Ultrastructure |
