| 摘要: |
| 采用紫外线(UV)灭活的同源精子通过冷休克抑制第二极体排放诱导黄姑鱼(Nibea albiflora)的雌核发育。结果表明, 黄姑鱼精子最适的灭活剂量为420mJ/cm2, 紫外照射剂量与黄姑鱼精子和卵子授精后的受精率及孵化率之间表现出明显的Hertwig效应。多次试验筛选的黄姑鱼雌核发育的最适条件为授精后2min于3℃处理8min。雌核发育仔鱼经形态学和流式细胞仪鉴定为二倍体。进一步利用10对微卫星分析了遗传物质在亲本和子代中的传递情况, 结果表明雌核发育后代的等位基因完全来自于母本, 没有父本基因参与; 雌核发育后代在5 个微卫星位点具有较高的重组率, 使其与母本保持了高度同质性 |
| 关键词: 黄姑鱼 雌核发育 人工诱导 紫外灭活 冷休克 微卫星 |
| DOI：10.11693/hyhz201302007007 |
| 分类号: |
| 基金项目:国家“863”计划, 2012AA10A413-5号; 浙江省重大科技专项, 2012C12907-8号 |
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| ARTIFICIAL INDUCTION OF DIPLOID GYNOGENESIS IN NIBEA ALIBIFLORAAND EVIDENCE FOR FEMALE HOMOGAMETY |
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XU Dong-Dong1, LOU Bao1, XUE Bao-Gui1, SHI Hui-Lai1, ZHAN Wei1, MA Shi-Lei1,2, MAO Guo-Min1
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1.Marine Fishery Institute of Zhejiang Province, Zhejiang Province Key Lab of Mariculture and Enhancement;2.Zhejiang Ocean University, College of Marine Science
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| Abstract: |
| The induction of diploid gynogenesis in Nibea albiflora was established using a combination of UV-irradiated homologous sperm and cold shock of the resulting embryo to inhibit the second meiotic division. The optimal UV irradiation of the N. albiflora sperm for gynogenesis was 420mJ/cm2. A typical “Hertwig effect” was observed based on the fertilization, hatching and occurrence of haploid by subjecting eggs fertilized with UV-irradiation sperm. Treatment optima for diploid gynogenesis were 3℃for 8min at 2min after fertilization based on experiments. Results obtained from the observations of external morphologies and detection of DNA content in larvae by flow cytometry proved that the larvae were diploid gynogenesis. Furthermore, genetic inheritance from the parents to the progeny was examined by 10 microsatellite loci. The genetic analysis revealed exclusively maternal inheritance in all gynogenetic fry and no paternal contributions were detected. The combination rate was relatively high at 5 microsatellite loci in the gynogenetic offspring, and thereby the offspring show high genetic homogeneity with their female parent. |
| Key words: Nibea albiflora gynogenesis artificial induction UV irradiation cold shock microsatellite |
