| 摘要: |
| 采用半静水生物测试法, 开展了Cr6+对日本黄姑鱼(Nibea japonica)的急性暴露试验, 研究了暴露6、12、24、72、96h后, Cr6+对日本黄姑鱼外周血细胞微核率、核异常率及肝胰腺超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性的影响。结果表明: 不同浓度Cr6+胁迫下, 红细胞微核率及核异常率均显著高于对照组(P<0.05); 相同浓度Cr6+胁迫下, 核异常率普遍高于微核率; 对照组CAT和SOD活性无明显变化(P>0.05), 不同浓度Cr6+处理组SOD和CAT活性均变化显著(P<0.05), 在96h内呈现出先升高、后降低、再升高的变化趋势。 |
| 关键词: Cr6+ 日本黄姑鱼 微核率 核异常率 超氧化物歧化酶 过氧化氢酶 |
| DOI：10.11693/hyhz201302010010 |
| 分类号: |
| 基金项目:国家高技术研究发展计划(863 计划)项目, SS2012AA100813 号 |
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| EFFECTS OF Cr6+ ON PERIPHERAL BLOOD CELL AND ANTIOXIDANT ENZYME ACTIVITY (SOD, CAT) OF NIBEA JAPONICA |
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ZHANG Cai-Ming1,2, CHEN Ying-Hua1, WU Chang-Wen1, LI Xiao-Ting1
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1.National Engineering Research Center of Marine Facilities Aquaculture, Marine Science School of Zhejiang Ocean University;2.Pingliang Normal School
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| Abstract: |
| An acute exposure experiment of Cr6+ on Nibea japonica was carried out in a semi-static biological test method. The abnormal rates of micronucleus and nuclear in peripheral blood cell, and the activities of superoxide dismutase (SOD) and catalase (CAT) in liver were determined in 6, 12, 24, 72 and 96h respectively. The results show that the erythrocyte micronucleus rates and nuclear abnormal rates exposed to Cr6+ solution at different concentrations were significantly higher than those in control group (P<0.05). The nucleus abnormal rates were commonly higher than micronucleus rate at the same Cr6+ concentration. The activities of SOD and CAT had no significant change in the control group during the experiment (P>0.05). Compared with the control group, activities of SOD and CAT had greatly changed in the test groups (P<0.05), which represented a tendency from increase todecrease and to increase again within 96 hours. |
| Key words: Cr6+ nibea japonica micronucleus rate nuclear abnormal rate superoxide dismutase (SOD) catalase (CAT) |
