| 摘要: |
| 采用聚丙烯酰胺不连续凝胶垂直电泳方法, 进行了中国北方沿海的河北渤海湾、山东莱州湾、山东胶州湾和江苏海州湾4个脊尾白虾(Exopalaemon carinicauda)地理群体间的生化遗传差异分析。结果表明, 12 种同工酶共有16个基因座位, 其中Ldh-1、Adh-1、Sdh-1、s-Mdh-1、Gcdh-1和Me-1共6个座位表现为多态; 平均每个座位等位基因有效值Ae= 1.2291?1.3009, 平均预期杂合度He= 0.1330?0.1675, 平均观察杂合度Ho= 0.1797?0.2474, 遗传距离D= 0.0050?0.0345; 聚类分析表明, 渤海湾群体和莱州湾群体亲缘关系最近, 其次为胶州湾群体与海州湾群体。本研究结果表明, 脊尾白虾遗传多样性处于较高水平, 种质资源状况良好, 有利于其种质资源的保护开发及遗传改良。 |
| 关键词: 脊尾白虾 地理群体 同工酶 遗传多样性 |
| DOI：10.11693/hyhz201302015015 |
| 分类号: |
| 基金项目:国家高技术研究发展计划(863)项目资助, “主要养殖甲壳类良种培育”, 2012AA10A409 号; 国家虾产业技术体系项目资助, CARS-47 号; 中央级公益性科研院所基本科研业务费资助项目, 20603022012021 号 |
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| BIOCHEMICAL GENETIC ANALYSIS OF DIFFERENT GEOGRAPHIC POPULATIONS FROM EXOPALAEMON CARINICAUDA |
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LI Ji-Tao, DUAN Ya-Fei, LI Jian, CHEN Ping, LIU Ping, GAO Bao-Quan
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Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences
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| Abstract: |
| The genetic variation of isozyme of Exopalaemon carinicauda was investigated using vertical polyacrylamide gel electrophoresis. Four geographic populations, i. e, distributed in Bohai Bay (BH), Shandong Laizhou Bay (LZ), Shandong Jiaozhou Bay (JZ), and Jiangsu Haizhou Bay (HZ) were collected to analyze the population diversity. Sixteen putative loci were detected from twelve enzymes, and six of them were polymorphic. The proportion of polymorphic loci (P0.99) of BH was 31.25%, and those of other three populations were all 37.50%. The mean effective number (Ae) of alleles per locus ranged from 1.2291 to 1.3009, and the mean values of observed (Ho) and expected (He) heterozygosities ranged from 0.1330 to 0.1675 and 0.1797 to 0.2474 respectively. The Nei’s genetic distance (D) among the four geographic populations ranged from 0.0050 to 0.0345, lower than that between subspecies. Cluster analysis indicated that BH and LZ populations were clustered together for the closest relationship, while JZ and HZ were clustered as an independent cluster. The results show that high level genetic diversity and good germplasm resources existed in E. carinicauda, which could contribute to its protection and genetic improvement in the future. |
| Key words: Exopalaemon carinicauda geographic population isozyme genetic diversity |
