| 摘要: |
| 以初始体重为(13.0±0.3)g 的斜带石斑鱼(Epinephelus coioides)为实验对象, 用铜添加量为0、1、3、5、10和20mg/kg的实验饲料投喂斜带石斑鱼8周, 研究斜带石斑鱼的铜需求量。结果表明, 饲料中添加0—20mg/kg铜对石斑鱼生长、饲料效率、存活率、形态学指标和红细胞比容没有显著性影响。随饲料铜水平的上升, 斜带石斑鱼全鱼和肝脏的铜含量呈先上升后平稳的趋势, 当饲料铜添加量达到或超过3mg/kg时, 石斑鱼全鱼和肝脏的铜含量趋于平稳。折线模型表明, 饲料中铜总含量为5—5.5mg/kg时, 石斑鱼全鱼和肝脏的铜含量能够达到饱和。当饲料中铜总含量≥6.00mg/kg时, 全鱼的铁、锰、锌含量显著下降。因此, 根据全鱼和肝脏铜含量的折线模型分析, 考虑到饲料铜对全鱼铁、锰、锌含量的影响, 作者认为当基础饲料铜含量为1.50mg/kg 时, 饲料中的最适铜添加量为3mg/kg, 饲料中铜总量为5—5.5mg/kg左右。 |
| 关键词: 斜带石斑鱼 铜 矿物质 |
| DOI：10.11693/hyhz201302031031 |
| 分类号: |
| 基金项目:国家科技支撑计划项目，2007BAD29B04 号; 国家自然科学基金项目, 31100296 号; 广东省重点实验室建设项目, 2009A060800019号; 广东省自然科学基金项目, SZ2011010005079 号; 惠州市科技计划项目, 2011B040010003 号。 |
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| DIETARY COPPER REQUIREMENT OF JUVENILE GROUPER EPINEPHELUS COIOIDES |
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YE Chao-Xia1, LIU Yong-Jian2, TIAN Li-Xia2, YANG Hui-Jun2, WANG An-Li1,2, ZHOU Li-Bin3
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1.Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University;2.School of Life Sciences, Sun Yat-sen University;3.Department of Life Science, Huizhou University
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| Abstract: |
| This study was conducted to investigate the copper requirement of juvenile grouper, Epinephelus coioides. Six diets were prepared by supplementing a casein-gelatin-based diet with 0, 1, 3, 5, 10 and 20mg/kg copper from CuSO4·5H2O. Grouper with an initial weight of (13.0±0.3)g were fed to satiation for 8 weeks. Growth performance, feed utilization, morphological indicators and hematocrit value of juvenile grouper were not significantly affected by dietary copper supplements ranging from 0 to 20mg/kg. Copper concentration of whole body and liver responded linearly to dietary copper supplement up to 3mg/kg and then reached a plateau. Broken-line analysis indicated that dietary copper of 5—5.5mg/kg satisfied whole body and liver copper storage. Whole body Fe, Mn and Zn were significantly lower when dietary total copper≥6mg/kg. Liver Fe, Mn and Zn were not significantly affected by dietary copper supplement ranging from 0 to 20mg/kg. Concerning broken-line analysis of whole body and liver Cu, and the interaction effect of Cu supplement on whole body Fe, Mn and Zn concentration, we estimate that copper supplement of 3mg/kg might be optimum to the basal diet containing 1.50mg/kg Cu, providing dietary total copper of 5—5.5mg/kg. |
| Key words: grouper Epinephelus coioides copper mineral |
