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野生重口裂腹鱼[Schizothorax(Racoma) davidi(Sauvage)]的性腺发育观察与人工繁殖研究
彭 淇, 吴 彬, 陈 斌, 冯 健
广西大学水产研究所
摘要:
对野生重口裂腹鱼[Schizothorax (Racoma) davidi (Sauvage)]的性腺发育观察与人工繁殖进行了研究。结果表明, 重口裂腹鱼雄鱼3龄达到性成熟, 而雌鱼5龄以上方达到性成熟, 其性腺指数随着年龄增加而显著上升(P<0.05); 雌鱼性腺指数、绝对怀卵量和卵径随着年龄和体重增加明显上升(P<0.05 or P<0.01)。对24尾5、6龄雌、雄重口裂腹鱼采用二针注射法进行人工催产实验, 平均采卵数为1.33万粒和2.02万粒, 采精量为25.4ml和34.8ml。其平均卵子受精率和受精卵出膜时间分别为74.2%和77.1%、203h和227h [水温(16.2±0.9)℃], 6龄雌鱼受精卵的卵径、卵重均明显高于5龄雌鱼, 6龄雌鱼的受精卵孵化率为41.9%, 明显高于5龄雌鱼的30.6% (P<0.05 or P<0.01), 选用年龄较大的雌鱼作为亲鱼, 是保证人工繁殖获得良好效果的关键。
关键词:  重口裂腹鱼, 性腺, 繁殖, 人工繁殖
DOI:10.11693/hyhz201303017017
分类号:
基金项目:广西科技厅基金资助计划项目, 2008-6-1-1 号
STUDY ON GONAD DEVELOPMENT AND PRELIMINARY ARTIFICIAL PROPAGATION OF WILD SCHIZOTHORAX (RACOMA) DAVIDI(SAUVAGE)
PENG Qi, WU Bin, CHEN Bin, FENG Jian
Institute of Aquaculture, Guangxi University
Abstract:
We studied the gonad development and preliminary artificial propagation of wild Schizothorax (Racoma) davidi (Sauvage) in experiment. We discovered that the 3-year.male.fish reached sexual maturation, but for female fish, it was 5-year.fish; the index of sexual gland in the fish increased apparently along with the age (P<0.05), the absolutely fecundity and egg size of female fish increased with age and weight obviously (P<0.05 or P<0.01). A test of artificial hasten parturition was proceeded to 24 brood stocks of 5-year.fish or 6-year.fish with twice injection method; the two brood stocks produces 13300 and 20200 eggs, 25.4mL and 34.8mL seminal fluids in average, the egg hatching rate 74.2% and 77.1%, insemination egg hatching time 203h and 227h [water temperature (16.2±0.9)℃], respectively. The egg size and egg weight that fertilized in brood stocks of age 6 are larger than those of age 5 obviously. The hatching rate of 6-year.fish was 41.9% greater than 30.6% of brood stocks of age 5 (P<0.05 or P<0.01). Therefore, choosing elder female fish as brood stock is important in artificial operation of fish reproduction.
Key words:  Schizothorax(Racoma) davidi(Sauvage)  Gonad  Reproduction  Artificial propagation
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