| 摘要: |
| 选取东海赤潮高发区常见的两种甲藻[东海原甲藻(Prorocentrum donghaiense)、塔玛亚历山大藻(Alexandrium tamarense)]和两种硅藻[中肋骨条藻(Skeletonema costatum)、海链藻(Thalassiosira sp.)], 以2-苯基乙胺、腐胺、亚精胺、精胺四种赤潮水体中常见的生物胺为因素, 设置0、5、25、100nmol/L四个浓度水平, 进行L16(45)正交添加培养实验。根据Logistic生长模型进行曲线拟合, 分析得到的生长参数。结果显示, 不同的生物胺对各赤潮藻生长影响的大小、趋势均存在差异。其中, 2-苯基乙胺对四种赤潮藻的生长影响最显著。高浓度的2-苯基乙胺对中肋骨条藻的生长具有明显的抑制作用, 对塔玛亚历山大藻的生长具有明显的促进作用。多胺物质(腐胺、亚精胺和精胺)对甲藻(东海原甲藻和塔玛亚历山大藻)生长的促进作用大于硅藻(海链藻和中肋骨条藻)。多胺中的亚精胺对塔玛亚历山大藻和海链藻的生长影响最大, 精胺对东海原甲藻和中肋骨条藻的生长影响最大。多胺可能是2010年东海赤潮由中肋骨条藻向东海原甲藻演替的一个诱导因素, 其中精胺可能发挥的作用 较大。 |
| 关键词: 生物胺 赤潮藻 正交实验 |
| DOI：10.11693/hyhz201303026026 |
| 分类号: |
| 基金项目:国家自然科学基金项目, 40976047 号 |
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| HOW THE BIOGENIC AMINES AFFECT HAB ALGAE’S GROWTH: A PRELIMINARY EXPLORATION |
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LIANG Cong-Cong1,2, ZHAO Wei-Hong1, MIAO Hui1
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1.Key Laboratory of Marine Ecology and Environmental Science Institute of Oceanology, Chinese Academy of Sciences;2.University of Chinese Academy of Sciences
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| Abstract: |
| Two species of dinoflagellates (Prorocentrum donghaiense, Alexandrium tamarense) and diatoms (Skeletonema costatum, Thalassiosira sp.) which are common in frequent-HAB area of East China Sea were selected in L16(45) orthogonal tests. Four biogenic amines, 2-phenylethylamine, putrescine, spermidine, and spermine, commonly detected in seawater during HAB time, were taken as test factors in four levels of 0, 5, 25, 100nmol/L. The nonlinear curve fitting with logistic growth model show that the influences varied in degree and trend when different biogenic amines acted on different HAB algae. Among them, 2-phenylethylamine was the most significant impact factor on the HAB algae’s growth. Spermidine affected A. tamarense and Thalassiosira sp. the most, while spermine had the biggest influence on P. donghaiense and S. costatum in three tested polyamines. Polyamines were considered as factors that affected the succession of HAB from S. costatum to P. donghaiense in East China Sea, 2010, of which spermine may be the dominating factor. |
| Key words: Biogenic amine HAB algae Orthogonal test |
