| 摘要: |
| 以金枪鱼碎肉为原料, 采用双酶分步水解法制备高F值酶解液, 通过Box-Behnken试验设计, 分别确定两步酶解的最佳条件, 酶解液经活性炭静态吸附去除游离芳香族氨基酸, 对脱芳后的酶解液进行氨基酸组成分析并测定F值。结果表明, 胃蛋白酶为第一步水解用酶, 酶解的最佳工艺条件为酶用量650U/g, 料水比1∶7(g/mL), 温度35.9℃; 风味蛋白酶为第二步水解用酶, 酶解的最佳工艺条件为酶用量50700U/g, pH 6.51, 温度51℃, 最终水解度达到36.87%±0.54%; 酶解液在pH 3.0, 温度35℃条件下, 经5%(质量体积分数)的活性炭吸附时间3h后, 脱芳率达到63.18%, F值为30.33, 符合高F值肽的要求。 |
| 关键词: 黄海西部沿岸冷水 黄海冷水团 青岛冷水团 中层冷水 陆架锋 化学水文学 |
| DOI：10.11693/hyhz201304010010 |
| 分类号: |
| 基金项目:我国近海海洋综合调查与评价专项课题, 908-01-ST03号; 中央级公益性科研院所基本科研业务费专项资金项目——黄海冷水团变异及其对海洋生态系统的影响, GY02-2010T05号。 |
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| CHEMICOHYDROGRAPHIC ANALYSIS OF THE ROLE OF THE YELLOW SEA WESTERN COASTAL COLD WATER IN FORMING THE SOUTHERN YELLOW SEA WESTERN BOTTOM COLD WATER AND ITS SEASONAL SUCCESSION |
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WEI Qin-Shen,YU Zhi-Gang,GE Ren-Feng,WANG Hui-Wu,WANG Bao-Dong
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1.Key Laboratory of Science and Engineering for Marine Ecological Environment, First Institute of Oceanography,
State Oceanic Administration;2.Key Laboratory of Marine
Chemistry Theory and Technology, Ministry of Education, Ocean University of China;3.Key Laboratory of Marine Science and Numerical Modeling,
First Institute of Oceanography, State Oceanic Administration
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| Abstract: |
| The preparation of high-Fischer ratio hydrolysates from the hydrolysis of tuna meat by two-step proteases was studied. Taking degree of hydrolysis (DH) as criterion, binary-enzymes hydrolysis process was optimized by response surface methodology. Hydrolysates derived from tuna meat were statically adsorbed by activated carbon in order to remove liberated aromatic amino acids (AAA). Detection of amino acid composition was carried out after treatment by activated carbon absorption. The results show that the optimum enzymolysis conditions selected by Design-expert 7.0 were as follows: pepsin was selected as the first enzyme, ratio of enzyme to substrate 650U/g, ratio of tuna meat to water 1∶7 (g/mL), temperature 35.9℃; flavourzyme was selected as the second enzyme, ratio of enzyme to substrate 50700U/g, pH 6.51, temperature 51℃, the highest degree of hydrolysis was 36.87%±0.54%. The optimal absorption condition by activated carbon was ratio of 1 : 20 (W/V), temperature 35℃, time 3h. The Fisher value of hydrolysates (removing AAA) determined on Automatic Amino Acid Analyzer was calculated to be 30.33, and a high AAA absorption ratio was 63.18%. |
| Key words: Yellow Sea western coastal cold water Yellow Sea Cold Water Mass Qingdao Cold Water Mass intermediate cold water continental shelf front chemicohydrography |
