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不同盐度下拟目乌贼(Sepia lycidas)胚胎发育过程研究
陈道海1, 郭永平2, 文 菁1, 梁汉清2
1.湛江师范学院环北部湾海洋药用动物资源保护与利用研究所,湛江师范学院生命科学与技术学院;2.湛江师范学院生命科学与技术学院
摘要:
分别配置盐度为13、18、23、28、33、38和43的海水, (23±0.5)℃温度条件下孵化拟目乌贼(Sepia lycidas)卵, 并对拟目乌贼胚胎发育情况进行观察与分析。结果表明, 拟目乌贼胚胎在盐度为28时发育最佳, 孵化历期为28—30天, 胚胎发育经过受精卵、卵裂期、囊胚期、原肠胚期、胚芽原基形成期、胚体形成期、漏斗形成期、眼点形成期、红眼期、心跳出现期、内壳发生期、色素形成期、墨囊形成期、黑眼期、内壳形成期和幼体出膜期等16个典型时期。盐度为13、18、38和43时, 发育停滞, 说明海水盐度是拟目乌贼胚胎发育过程中一个很重要的影响因素。
关键词:  拟目乌贼  盐度  胚胎发育
DOI:10.11693/hyhz201305023023
分类号:
基金项目:国家星火计划项目, 2012GA780020号; 广西科技合作与交流计划项目, 桂科合1346011-25号; 广东省教育部产学研合作专项, 2011B090400274号; 广东省海洋渔业技术推广专项, A200899E01号; 湛江市科技攻关项目, 2011D02-44号。
SALINITY IMPACT ON EMBRYONIC DEVELOPMENT OF CUTTLEFISH SEPIA LYCIDAS
CHEN Dao-Hai1, GUO Yong-Ping2, WEN Jing1, LIANG Han-Qing2
1.Zhanjiang Normal University, Round Beibu Gulf Institute for the Protection and Utilization of Marine Animals in Medicine,Zhanjiang Normal University, College of Life Science and Technology;2.Zhanjiang Normal University, College of Life Science and Technology
Abstract:
To understand the salinity impact on embryonic development of cuttlefish Sepia lycidas, we carried out an experiment at water temperature (23±0.5)℃ in pH 7.41 in salinities of 13, 18, 23, 28, 33, 38 and 43. The results show that the most suitable salinity was 28, and the peak time of hatching was 28—30d. The embryonic development was divided into 16 stages: cleavage stage, blastula stage, gastrulae stage, organ development stage, embryo growth stage, and so on. The embryonic development failed at salinity 13, 18, 38 and 43. Therefore, the salinity is an important environmental factor for S. lycidas early development.
Key words:  Sepia lycidas  salinity  embryonic development
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