| 摘要: |
| 采用藻类二氧化碳浓缩机制(CO2 Concentrating Mechanism, CCM)关键酶胞外碳酸酐酶(periplasmic Carbonic Anhydrase, pCA)的抑制剂AZ (acetazolamide)、DIDS (4’4’-diisothiocyana to silbene-2, 2-disulfonic acid)和SITS (4-acetamido-4’-isothiocyano-2, 2’-stibene-disulfonate)等对不同生长阶段的坛紫菜(Pyropia haitanensis)叶状体进行处理, 利用pH-drift技术研究了高温胁迫下坛紫菜利用无机碳的特点。结果表明: (1) 坛紫菜主要依赖pCA利用HCO3?, 且能力较强; (2) 高温抑制坛紫菜吸收利用无机碳, 温度越高影响越严重; (3) 坛紫菜未性成熟组叶片对无机碳的利用能力远大于成熟组, 但对高温胁迫的抗性低于成熟组。高温造成藻体细胞膜系统损伤和脂肪酸成分变化或许是高温抑制藻类CCM的原因之一。 |
| 关键词: 坛紫菜 高温胁迫 二氧化碳浓缩机制(CCM) |
| DOI：10.11693/hyhz201305038038 |
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| 基金项目:国家自然科学基金项目, 40776077号; 浙江省自然科学基金项目, LY12D06003号; 宁波市海洋藻类资源高效开发利用创新团队项目, 2011B81007号; 浙江省海水养殖科技创新团队项目, 2010R50025号; 浙江省海洋生物技术产业科技创新团队项目, 2010R50029号。 |
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| UTILIZATION OF INORGANIC CARBON IN PYROPIA HAITANENSIS (RHODOPHYTA) UNDER HEAT STRESS |
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WANG Shu-Gang, YANG Rui1, ZHOU Xin-Qian, SONG Dan-Dan, SUN Xue, LUO Qi-Jun
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School of Marine Science, Ningbo University, Key Laboratory of Applied Marine Biotechnology, Ministry of Education,Marine Biotechnology Laboratory, Ningbo University
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| Abstract: |
| Mechanisms of inorganic carbon utilization by red seaweed Pyropia haitanensis (Rhodophyta) were studied by pH-drift technique in a closed system. Inhibitors of periplasmic Carbonic Anhydras (pCA), the key enzyme in CO2 Concentrating Mechanism (CCM), such as AZ, (acetazolamide), DIDS (4’4’-diisothiocyanatosilbene-2, 2-disulfonic acid) and SITS (4-acetamido-4’-isothiocyano-2, 2’-stibene-disulfonate) were applied to detect the CCM in P. haitanensis under heat stress. The results indicate that: (1) P. haitanensis had a strong ability to utilize HCO3?, which was mainly conducted by pCA; (2) Heat stress inhibited the utilization of inorganic carbon (Ci) of P. haitanensis, and the higher the temperature, the lower utilization; (3) The thalli of the immature group had higher CCM efficiency and lower heat tolerance than the mature groups. The breakdown of membrane systems and the changes in the components of aliphatic acid in algae cells might be the reason of the CCM inhibition under heat stress. |
| Key words: Pyropia haitanensis heat stress CO2 Concentrating Mechanism (CCM) |
