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我国重要帘蛤科(Veneridae)贝类的16S rRNA序列系统学分析
赵 婷1, 吴 琪2, 潘宝平1
1.天津师范大学生命科学学院 天津市动植物抗性重点实验室;2.中国科学院海洋研究所
摘要:
本文对我国隶属于帘蛤科(Veneridae)10个亚科、17个属、20种贝类的16S rRNA基因片段进行了系统学分析, 上述动物的16S rRNA片段长度在438—648bp之间, 利用PAUP软件包在对序列比对基础上构建了邻接系统树(NJ)和最大拟然系统树(ML)。16S rRNA数据显示, 我国帘蛤科贝类由三个主要分支组成, 美女蛤亚科中的加夫蛤属(Gafrarium)可能是一个单型属, 该属与美女蛤属合并为加夫蛤属比较恰当。帘蛤亚科与雪蛤亚科应属于不连续的分类单元。另外, 青蛤亚科与仙女蛤亚科均应作为独立的亚科存在。本文的研究结论与修订后的帘蛤科形态分类观点一致。
关键词:  双壳类  帘蛤科  16S rRNA基因  分子系统学
DOI:10.11693/hyhz201306013
分类号:
基金项目:天津市科委应用基础与前沿技术重点项目资助, 12JCZDJC22800号, 09JCZDJC19300号。
MOLECULAR PHYLOGENY OF VENERIDAE (MOLLUSCA, BIVALVIA) BASED ON 16S rRNA SEQUENCES
ZHAO Ting1, WU Qi2, PAN Bao-Ping1
1.College of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University;2.Institute of Oceanology, Chinese Academy of Sciences
Abstract:
We conducted a phylogeny analysis on partial 16S rRNA genes among 20 species in Veneridae under 17 genera and 10 subfamilies. The fragments of 16S the rRNA were between 438bp to 648bp. The trees of Neighbor-joining (NJ) and Maximum-likelihood (ML) were established with PAUP beta based on sequence alignment. The 16S rRNA data present three major clades. Gafrariumis a monotype grouping of Circinae and Circeand Gafrariumshould be combined into genus Gafrarium. Subfamilies of Chioninae and Venerinae should be discrete taxa. Moreover, Callistinae and Cyclininae should be distinct subfamilies from other taxon. Results of this study showed that there were consistencies with the modified morphologic classification of Veneridae.
Key words:  Bivalvia  Veneridae  16S rRNA gene  molecular phylogeny
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