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三疣梭子蟹(Portunus trituberculatus)幼蟹饲料中胆固醇的适宜添加量研究
韩 涛1, 王骥腾2, 胡水鑫2, 李新宇2, 李荣华3, 母昌考3, 王春琳3
1.浙江海洋学院水产学院, 宁波大学海洋学院;2.浙江海洋学院水产学院;3.宁波大学海洋学院
摘要:
采用单因素实验设计方法, 以初始体重为20g左右的三疣梭子蟹(Portunus trituberculatus)幼蟹为实验对象, 胆固醇添加量为0.003%、0.3339%、0.655%、0.986%、1.303%和1.628%的实验饲料投喂8周, 研究其胆固醇需求量。结果表明, 饲料中添加0.003%—1.628%胆固醇对各处理组的存 活率无显著性影响(P>0.05)。随饲料胆固醇水平的上升, 三疣梭子蟹幼蟹的增重率呈先上升后下降的趋势, 当饲料胆固醇水平≥0.655%时, 三疣梭子蟹幼蟹的增重率呈下降趋势。折线模型表明, 饲料中胆固醇添加量为0.6%时, 三疣梭子蟹幼蟹的增重率、性腺指数和壳宽增加达到最高值。根据增重率折线模型结果, 作者认为三疣梭子蟹幼蟹饲料中添加0.6%左右胆固醇较为适宜
关键词:  三疣梭子蟹  胆固醇  折线模型  需要量
DOI:10.11693/hyhz201306017
分类号:
基金项目:浙江省教育厅科研计划项目, Y201225349 号; 浙江省水产育种专项, 2012C12907-3 号; 浙江省海水养殖重点科技创新团队项目, 2010R50025-08 号; 国家星火计划项目, 2013GA701041 号, 2013GA701002 号; 浙江省科技厅公益性技术应用研究项目, 2013C32037号, 2013C31032号; 浙江省海洋与渔业局海洋经济和渔业新兴产业补助项目, 2011年; 舟山市科学技术局一般海洋类项目, 2013C41007 号; 宁波市海洋蟹类产业科技创新团队项目, 2011B81003 号; 宁波市重大科技攻关计划项目, 2013C11017 号; 王宽诚基金资助。
NEEDS OF DIETARY CHOLESTEROL FOR JUVENILE SWIMMING CRAB PORTUNUS TRITUBERCULATUS
HAN Tao,WANG Ji-Teng,HU Shui-Xin,LI Xin-Yu,LI Rong-Hua,MU Chang-Kao,WANG Chun-Lin
1.Fisheries College of Zhejiang Ocean University;2.School of Marine Science of Ningbo University
Abstract:
To investigate cholesterol requirement by juvenile swimming crab Portunus trituberculatus, six diet groups were set up in one-way analysis model for adding 0.003%, 0.3339%, 0.655%, 0.986%, 1.303%, and 1.628% cholesterol into diet, respectively. Swimming crabs in initial weight about 20g were fed for 8 weeks. Results show that at the end of the experiment, the survival rate was not affected significantly by cholesterol enrichment in all six groups. Weight gain responded linearly to dietary cholesterol supplement up to 0.655% and then went down. Broken-line analysis indicated that dietary cholesterol of 0.6% satisfied the growth performance. At dietary cholesterol supplement 0.655%, crab had the highest weight gain, gland sex index, and carapace width gain. Therefore, we believe that, the optimal dietary cholesterol requirement by juvenile swimming crab was approximately 0.6%.
Key words:  swimming crab Portunus trituberculatus  cholesterol  broken-line  requirement
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