| 摘要: |
| 采用高浓度无机三态氮(铵氮NH4+-N、亚硝氮NO2--N和硝氮NO3--N)共存的模拟海水体系, 在最适生长条件下, 研究了小分子有机物(糖类、有机酸、醇、有机氮)和pH对海洋着色菌(Marichromatium gracile) YL28去除水体无机三态氮的影响。结果表明: 以葡萄糖、乙酸钠和乙醇为唯一碳源时, 水体中的高浓度NO2--N和NO3--N均能被完全去除, NH4+-N的去除率分别为93.40%、84.55%和66.63%; 碳源为乙酸钠时菌体生长最好, 体系中添加蛋白胨或尿素, 仅NH4+-N的去除效果明显降低。pH值在6.0 - 9.0时, 该菌株对NH4+-N、NO2--N和NO3--N均具有去除能力。由此可知: YL28菌株对模拟海水养殖水体中高浓度无机三态氮具有良好的去除能力, 高浓度有机氮化物(蛋白胨和尿素)对NH4+-N的去除能力有明显影响, 但对NO2--N和NO3--N仍保持高效的去除能力。本研究为不产氧光合细菌制剂在水产养殖中的合理应用提供参考。 |
| 关键词: 海洋着色菌 铵氮 亚硝氮 硝氮 |
| DOI：10.11693/hyhz20131200211 |
| 分类号: |
| 基金项目:国家自然科学基金项目, 31070054 号, 31270106 号; 国家海洋公益性行业科研专项, 201505026 号; 福建省自然科学基金项目, 2012 J01136 号 |
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| INFLUENCES OF LOW-MOLECULAR-WEIGHT ORGANIC CARBON AND NITROGEN SOURCES ON GROWTH AND INORGANIC NITROGEN REMOVAL BY MARICHROMATIUM GRACILE STRAIN YL28 |
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JIANG Peng,ZHAO Chun-Gui and YANG Su-Ping
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Department of Bioengineering and Biotechnology,Huaqiao University,Fujian,Department of Bioengineering and Biotechnology,Huaqiao University,Fujian,Department of Bioengineering and Biotechnology,Huaqiao University,Fujian
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| Abstract: |
| The effects of low-molecular-weight sugar, organic acid, alcohol, and organic nitrogen, as well as pH on the removal of high-concentration inorganic nitrogen from simulated seawater were investigated by purple sulfur bacteria Marichromatium gracile strain YL28. The results show that high-concentration of nitrate and nitrite could be completely removed from simulated wastewater with strain YL28 when grown on glucose, sodium acetate, and ethanol as the sole carbon source, the removal rate of ammonium reached 93.40%, 84.55%, and 66.63%, respectively. Sodium acetate was the best carbon source for the cell growth. The removal efficiency to ammonium by the strain was apparently decreased in the presence of peptone or urea. The optimal pH for the removal of ammonium, nitrite, and nitrate was in the range of 6.0 to 9.0. These results suggest that strain YL28 is capable of removing high-concentration inorganic nitrogen from mariculture wastewater; and the removal capacity to ammonium with the strain was markedly affected by high-concentration organic nitrogen compounds such as peptone and urea, whereas the removal to nitrite and nitrate kept at a high level. This study would be applicable for treating wastewater with anoxygenic phototrophic bacteria in aquaculture. |
| Key words: Marichromatium gracile ammonium nitrate nitrite |
