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饲料添加不同脂肪及蛋白质水平对日本沼虾(Macrobrachium nipponensis)生长性能、体成分及消化酶活力的影响
斯烈刚,邹李昶,申屠基康,朱卫东
宁波市海洋与渔业研究院 宁波,余姚市水产技术推广中心 余姚,宁波市海洋与渔业研究院 宁波,余姚市水产技术推广中心 余姚
摘要:
本实验以64d为限, 设A1(34%), A2(38%), A3(42%)三个蛋白梯度水平和B1(5%), B2(8%), B3(11%)三个脂肪梯度水平, 采用正交试验方法研究了饲料中不同脂肪及蛋白质水平对日本沼虾(Macrobrachium nipponensis) [平均体重(0.69±0.23)g]生长性能、体成分和消化酶活力的影响。结果表明, 脂肪和蛋白质水平对日本沼虾成活率影响不显著(P>0.05), 脂肪水平对32d相对增重率和特定生长率影响显著(P<0.05)。B3组的特定生长率和相对增重率均显著低于B1和B2组(P<0.05)。全虾和 肌肉的脂肪含量显著地受饲料脂肪水平的影响(P<0.05)。饲料中蛋白质水平对全虾的干物质、蛋白质和灰分含量, 以及肌肉中脂肪、蛋白质含量影响显著(P<0.05)。饲料脂肪水平对肝胰脏中脂肪酶活力影响显著(P<0.05), 蛋白水平对肝胰脏中脂肪酶、蛋白酶和淀粉酶活力影响不显著(P>0.05)。本文认为日本沼虾饲料脂肪水平应以5%—8%较为合适, 蛋白质水平应在38%左右较为合适。
关键词:  日本沼虾  配合饲料  脂肪  蛋白质  生长  体成分  消化酶
DOI:10.11693/hyhz20140300087
分类号:
基金项目:浙江省重大科技专项农业重点项目, 2008C12083号; 国家科技部星火计划项目, 2010GA701077号; 宁波市农业领域科技重大攻关择优委托项目, 2012C10032 号。
EFFECT ON DIFFERENT DIETARY LIPID AND PROTEIN LEVEL ON GROWTH PERFORMANCE, BODY COMPOSITION AND DIGESTIVE ENZYMES ACTIVITIES OF FRESHWATER SHRIMP MACROBRACHIUM NIPPONENSIS
SiLiegang,Zou Lichang,SHENTU Jikang and ZHU Weidong
Ningbo Academy of Oceanology and Fishery,Fishery Technology Extension Center of Yuyao,Ningbo Academy of Oceanology and Fishery,Fishery Technology Extension Center of Yuyao
Abstract:
A bifactorial model was used to investigates the effect of dietary lipid and protein on growth performance, body composition and digestive enzymes activities of freshwater shrimp Macrobrachium nipponensis. Nine groups of M. nipponensiswith three replicates and 10 shrimp [average weight (0.69±0.23)g] per replicate were fed nine experimental diets containing three lipid levels (5%, 8% and 11%) and three protein levels (34%, 38% and 42%) for 64 days. The result showed that survival rate (SR) of M. nipponensiswere not affected significantly (P>0.05) by dietary lipid and protein. Weight gain (WG) and specific growth rate (SGR) of 32 days for M. nipponensiswere affected significantly (P<0.05) by dietary lipid. When grouped by lipid levels, the SGR and WG of M. nipponensisfed the diets B3 were lower than that of shrimp fed the diets B1and B2(P<0.05). Whole-body and muscle crude lipid (EF) were affected significantly (P<0.05) by dietary lipid. Proximate analysis indicated that whole-body dry matter (DM), crude protein (CP) and ash contents or muscle CP and EF contents were affected significantly (P<0.05) by dietary protein. Hepatopancreas lipase activities were affected significantly (P<0.05) by dietary lipid. Hepatopancreas protease, lipase and amylase activities were not affected significantly (P>0.05) by dietary protein. In conclusion, positive impacts of optimizing protein and lipid component in terms of economic and environmental concerns, diet containing lipid 5%—8% and protein 38% with protein was beneficial consequences of further refinement of commercial M. nipponensisproduction feed.
Key words:  Macrobrachium nipponensis  dietary  lipid  protein  growth  body composition  digestive enzymes
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