| 摘要: |
| 谷胱甘肽硫转移酶是一类在细胞解毒和抗氧化过程中发挥重要作用的超家族酶系。采用cDNA末端快速扩增技术, 从菲律宾蛤仔中克隆获得Rho 型GST和微粒体型GST基因的全长序列(分别命名为VpGSTR 和VpGSTMi)。序列分析显示, VpGSTR 和VpGSTMi 的cDNA 全长分别为942bp和661bp, 编码234 和149 个氨基酸。系统分析结果表明, 菲律宾蛤仔GSTR 与GSTMi 在进化树上的位置与其分类所处的位置一致。荧光定量PCR 结果发现, VpGSTR 和VpGSTMi 广泛分布于所检测组织中, 且在肝胰腺组织中表达量最高。在鳗弧菌侵染后, VpGSTR 和VpGSTMi 基因表达量在24h内均表现出明显的上升趋势。上述结果表明, VpGSTR 和VpGSTMi 可能在菲律宾蛤仔抵御细菌侵染导致的氧化应激中发挥重要功能。 |
| 关键词: 谷胱甘肽硫转移酶 菲律宾蛤仔 基因克隆 基因表达 |
| DOI：10.11693/hyhz20130700102 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目,41206105号。 |
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| SEQUENCE AND TRANSCRIPTIONAL PROFILES OF RHO AND MICROSOMAL CLASS GST GENES IN MANILA CLAM VENERUPIS PHILIPPINARUM |
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ZHANG Ying-Ying1,2, YUAN Ze-Yi3, WANG Qing1, LI Qiao-Mei1,2, WU Hui-Feng1, ZHAO Jian-Min1
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1.Yantai Institute of Coastal Zone Research, Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Chinese Academy of Sciences, Yantai 264003, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.National Marine Data and Information Service, Tianjin 300171, China
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| Abstract: |
| Glutathione S-transferase (GST) is a family of versatile enzymes playing significant roles in cellular defense against oxidative stress. In this study, the full-length cDNAs of two glutathione S-transferases (GSTs) (named VpGSTR and VpGSTMi) were cloned from Venerupis philippinarum using RACE technique. Sequence analysis showed that VpGSTR comprised an open reading frame (ORF) of 705bp, encoding a polypeptide of 234 amino acids, and VpGSTMi possessed an ORF of 450bp, encoding a polypeptide of 149 amino acids. Phylogenetic analysis showed that VpGSTR and VpGSTMi were linked in evolution with other Rho and microsomal homologues. The VpGSTR and VpGSTMi transcript were detected in all tissues but in the highest level in hepatopancreas. After challenged with Vibrio anguillarum, the expression of both VpGSTR and VpGSTMi transcripts was up-regulated in 24h. These findings suggest that VpGSTR and VpGSTMi may play an important role in cellular defense against oxidative stress caused by bacterial challenge. |
| Key words: glutathione S-transferases Venerupis philippinarum gene cloning expression analysis |
