| 摘要: |
| 采用活性炭吸附法脱除鱿鱼(Ommastrephes bartrami)皮胶原蛋白提取液中色素, 优化脱色条件, 以进一步利用鱿鱼皮胶原蛋白。在确定该色素特征吸收波长为420 nm 条件下, 分别研究了活性炭添加量、脱色时间、溶液pH 值和脱色温度对鱿鱼皮胶原蛋白液色素脱除效果影响。在单因素实验基础上, 选择脱色时间30 min, 进行活性炭添加量、溶液pH 值、脱色温度三因素三水平Box-Behnken 中心组合响应面分析试验。结果表明, 活性炭吸附鱿鱼皮胶原蛋白液色素最佳条件为活性炭添加量4.2%, 溶液pH 值2.27, 脱色温度35℃, 该条件下鱿鱼皮胶原蛋白液的脱色率为45.6%, 蛋白质和氨基酸损耗率分别为19.65%和22.93%。脱色后鱿鱼皮胶原蛋白的甘氨酸含量最高(136.02 mg/g, 相对含量23.05%), 谷氨酸、精氨酸和脯氨酸含量也较高, 而半胱氨酸和蛋氨酸总量不到1%, 符合水产蛋白氨基酸和I 型胶原蛋白氨基酸特点。 |
| 关键词: 鱿鱼皮 胶原蛋白提取液 色素 活性炭 脱色 |
| DOI：10.11693/hyhz20141000297 |
| 分类号: |
| 基金项目:国家星火计划项目, 2013GA700260号; 浙江省厅市会商项目, 2013C02003号; 舟山市科技局项目, 2013C41008号。 |
|
| OPTIMIZATION OF DECOLORING FOR COLLAGEN EXTRACT FROM SQUID (OMMASTREPHES BARTRAMI) SKIN WITH ACTIVATED CARBON |
|
ZHANG Xue, LINGHU Qing-Qing, TONG Xiao-Qian, MAO Gui-Zhu, LUO Hong-Yu, SONG Ru
|
|
Zhejiang Provincial Key Laboratory of Health Risk Factors for Seafood, College of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China
|
| Abstract: |
| The color of collagen extract from squid (Ommastrephes bartrami) skin is brown-red, which may be an unwanted property in food industry, to which we use activated carbon to absorb the pigment of collagen extract from squid skin. Results show that the pigment demonstrated characterized absorbance at 420 nm. The effects of independent variables on the decoloring of collagen extract, including activated carbon addition content, decoloring time, pH value of solution, and absorbing temperature were evaluated. Three independent variables including activated carbon addition content, pH value, and absorbing temperature were selected in the response surface method in the Box-Behnken design under decoloring time of 30 min. Results show that the optimal absorbance conditions for decoloring of collagen extract were: activated carbon addition content 4.2%, pH 2.27, and temperature 35 ℃. The decoloring ratio was 45.6%. Moreover, the loss rates were 19.65% for protein and 22.93% for amino acid in the decoloring process. The glycine content was the highest (136.020mg/g, relative content 23.05%), and the levels of glutamic acid, arginine, and proline were relatively high as indicated in amino acid composition analysis. In addition, the total content of cysteine and methionine was below 1%. Amino acid analysis indicated that the squid skin collagen after decolorization was in accordance with the aquatic collagen and type I collagen characteristics. |
| Key words: squid (Ommastrephes bartrami) skin collagen extract pigment activated carbon decoloring |
