| 摘要: |
| 为探讨DNA条形码基因COⅠ序列在石鲈亚科鱼类物种鉴定的有效性,本研究分析了印度-太平洋区域的石鲈亚科7个属29种鱼类9个个体长度为651 bp的COⅠ基因序列,利用MEGA 5.0计算石鲈亚科种内与种间的遗传距离,并基于最大简约法与最大似然法构建了其分子系统进化关系。结果显示:石鲈亚科鱼类种间遗传距离在0.021-0.240之间,平均遗传距离0.184;种内遗传距离在0.000-0.009之间,平均0.004。其中种间平均遗传距离(0.184)显著大于种内平均遗传距离(0.004),种间遗传距离是种内的46倍。同时,各物种间遗传距离均大于Hebert推荐区分物种的最小种间遗传距离0.02(2%)。基于COⅠ序列构建的系统进化树,同一物种不同个体间均能聚成独立的单系,表明COⅠ基因可作为石鲈亚科物种准确鉴定的有效条形码基因。同时,系统进化树上,石鲈属是非单系类群,主要形成5个分支,而不同分支的种类与其地理分布存在一定的相关性,与近年部分分子系统地理学的研究观点一致。 |
| 关键词: DNA 条形码 COⅠ基因 胡椒鲷亚科 系统分类 |
| DOI：10.11693/hyhz20150200059 |
| 分类号:Q953 |
| 基金项目:广东省教育厅青年创新人才类项目,2014KQNCX164号;广东省大学生创新创业训练计划项目,201511347028号;仲恺农业工程学院大学生创新基金项目,2015A08号,2015A10号。 |
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| DNA BARCODING FOR WORLDWIDE HAEMULINAE IN CLASSIFICATION AND PHYLOGENY |
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Liang Rishen,Wu Liyu,Li Jinxiang,Cheng Shuo and Xie Wenfeng
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College of Life Science,College of Life Science,College of Life Science,College of Life Science,College of Life Science
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| Abstract: |
| To investigate the feasibility of using COⅠ sequences as DNA barcodes to identify Haemulinae species, we determined the 651 bp COⅠ sequences of 91 Haemulinae individuals sampled from 7 genera 29 species, calculated the genetic distances inter- and intra-species using MEGA 5.0, and constructed the molecular phylogenetic trees using maximum parsimony and maximum likelihood methods. The result show that the inter-species genetic distance was 0.021-0.240 in average of 0.184, while that of intra-species was 0.000-0.009 in average of 0.004. The average inter-species value (0.184) is 46 times larger than that of intra-species one (0.004). Moreover, each inter-species genetic distance value is larger than the minimum species identification value 0.200 (2%) suggested by Hebert. In the molecular phylogenetic trees, individuals of each species formed an independent monophyletic group, indicating that COⅠ could be used as an effective barcode gene for accurate identification among Haemulinae species. Meanwhile, as the phylogenetic trees show, the Pomadasys fish are non-monophyletic and form 5 independent groups. Such phylogenetic relationships are due to their geographic distribution, which is consistent with the viewpoint of modern molecular phylogeography studies. |
| Key words: DNA barcodes COⅠ gene Haemulinae phylogeny |
