| 摘要: |
| 以采自海南海口、广西防城港、广西北海、广东湛江、福建漳州、山东荣成、山东威海7个地理群体62 个泥蚶个体为材料, 获取592bp 线粒体COI 基因片段序列, 并进行遗传多样性及分化分析。多态性遗传参数统计显示: 62 个个体共检测出103 个多态性位点, 定义了26 个单倍型; 总群体单倍型多样性指数为0.834, 核苷酸多样性指数为0.01665, 平均核苷酸差异数为9.85772。7 个群体均显示出较丰富的遗传多样性, 群体内遗传距离及群体内遗传多样性参数显示中国沿海泥蚶遗传多样性由北向南呈升高趋势, 而群体内遗传分化系数也呈现升高的趋势。基于26 个单倍型COI 序列构建的NJ 树和UPGMA 树以及基于群体间遗传距离构建的UPGMA 树显示, 荣成群体和威海群体亲缘关系较近, 聚成一小支, 而后与漳州群体相聚, 然而南方类群并没有聚为独立的一支。 |
| 关键词: 泥蚶 COI 遗传多样性 遗传分化 |
| DOI：10.11693/hyhz20140400105 |
| 分类号: |
| 基金项目:国家自然科学基金项目,40406032号,40876084号,31000948号;中国科学院战略性先导科技专项,XDA1102030304号;中国科学院海洋研究所“一三五”生物多样性项目,2012IO060101号;海洋公益性行业科研专项经费项目,200805069号;国家环境保护部项目,物种10-二-14号。 |
|
| GENETIC DIVERSITY OF DIFFERENT POPULATIONS OF TEGILLLARCA GRANOSA IN COASTAL WATERS OF CHINA BASED ON PARTIAL COI GENE SEQUENCES |
|
LIU Chun-Fang1,2, LI Cui1,2, ZHANG Zhen1, WANG Hai-Yan1
|
|
1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China
|
| Abstract: |
| We collected 62 Tegillarca granosa samples from seven populations i.e., South China in Haikou, Fangchenggang, Beihai, Zhanjiang, and Zhangzhou, and North China in Rongcheng and Weihai in coastal waters, from which 592bp nucleotide sequences of mitochondrial COI gene fragments of T. granosa were obtained, and 103 variable nucleotide positions and 26 haplotypes were revealed in the seven populations. The haplotype diversity (Hd) was 0.834, the nucleotide diversity (Pi) was 0.01665, and the average nucleotide difference (K) was 9.85772. Results show high genetic diversity within populations. The genetic distances and genetic diversity parameters within populations suggested that genetic diversity and genetic differentiation coefficient within T. granosa populations in coastal waters of China increased north to south generally. The dendrograms in the neighbor-joining method based on the sequences of the 26 haplotypes and in the UPGMA (Unweighted Pair Group Method with Arithmetic Mean) method based on genetic distances show close relationship between Rongcheng and Weihai, and then the two populations join a clade with Zhangzhou population, while the southern groups did not form any independent clade. |
| Key words: Tegillarca granosa COI genetic diversity genetic differentiation |
