| 摘要: |
| 将大菱鲆(Scophthalmus maximus)从正常养殖水温18℃ 快速连续降温至1℃, 并在18、13、8、5、3 和1℃ 共计6 个温度点取血采样, 分别测定血清中的谷丙转氨酶(ALT)、肌酐(CR)、乳酸脱氢酶(LDH)、血糖(GLU)、皮质醇以及不同组织中Wap65-1 基因的表达量。结果发现, 随着温度降低,大菱鲆的血清ALT 活性呈升高的趋势, 且在8—1℃ 区间内显著高于18—13℃ 区间(P<0.05); 血清CR 浓度呈升高的趋势, 在3℃ 时最高, 且显著高于除1℃ 之外的其它实验(P<0.05); 血清中LDH 活性呈先升高后降低的趋势, 在13—5℃ 区间内显著高于其它实验组(P<0.05); 血清GLU 浓度呈先升高后降低的趋势, 在8—3℃ 区间内显著高于其它实验组(P<0.05); 血清皮质醇浓度呈升高的趋势,在1℃ 时最高, 且显著高于其它实验组(P<0.05)。连续降温过程中, 大菱鲆所有组织Wap65-1 基因在特定温度下表达量都有上调(脑、胃、肝脏、头肾、肾脏、脾脏、肌肉、肠道、心脏)。其中, 脑、胃、头肾、肾脏和心脏组织中的Wap65-1 基因在正常温度下基本不表达, 只在降温过程中表达; 肝脏、脾脏、肠道和肌肉这4 种组织中的Wap65-1 基因在正常温度下有所表达, 且在降温过程中表达量显著升高(P<0.05)。 |
| 关键词: 大菱鲆 连续降温 血清生化指标 Wap65-1 基因表达 |
| DOI：10.11693/hyhz20140400115 |
| 分类号: |
| 基金项目:青岛市应用基础研究(青年专项)课题,14-2-4-59-jch号;山东省科技发展计划课题,2011GGC21007号;中科院知识创新工程重要方向项目,KZCX2-EW-Q215号。 |
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| EFFECT OF CONTINUOUS COOLING ON SERUM BIOCHEMICAL PARAMETERS AND Wap65-1 EXPRESSION OF ADULT TURBOT SCOPHTHALMUS MAXIMUS |
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JI Li-Qin1,2, ZHANG Ming-Ming1,2, WANG Lei1, WANG Bao-Jie1, LIU Mei1, HAN Long-Jiang1,2, JIANG Ke-Yong1
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1.National & Local Joint Engineering Laboratory for Ecological Mariculture, Institute of Oceanology, China Academy of Sciences, Qingdao 266071, China;2.Fisheries College, Ocean University of China, Qingdao 266003, China
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| Abstract: |
| Adult turbots were subject to continuous cooling from 18 to 1℃, and five individuals were collected at each specific temperature including 18, 13, 8, 5, 3, and 1℃. Contents of five serum biochemical indices were measured consisting of ALT, CR, LDH, GLU, cortisol and the expression of Wap65-1 gene in different tissues was tested. The results indicate that as temperature decreased, the serum ALT activity increased and groups from 8℃ to 1℃ were significantly higher than 18℃ to 13℃ (P<0.05). The concentration of serum CR increased as temperature decreased, and was the highest at 3℃, which was significantly higher than those of other groups except for 1℃ group (P<0.05). Serum LDH activity increased and then decreased, and in the range from 13℃ to 5℃ it was significantly higher than those of other groups. Serum GLU concentration increased and then decreased, and in the range from 8℃ to 3℃ it was significantly higher than the other groups. Serum cortisol concentration increased as the temperature decreased, and was the highest at 1℃ which was significantly higher than those of other groups (P<0.05). The expression of Wap65-1 showed that the continuous cooling had an effect on all tissues (brain, stomach, liver, head kidney, kidney, spleen, muscle, intestine, and heart at specific temperature). The expression of Wap65-1 in them increased significantly during cooling. Wap65-1 of the brain tissue, stomach tissue, head kidney, kidney and heart tissue at normal temperatures had almost no expression, but expressed significantly during the cooling process. Wap65-1 of liver, spleen, intestine, and muscle tissues expressed at normal temperature and expression levels elevated significantly in the cooling process. |
| Key words: turbot Scophthalmus maximus continuous cooling serum biochemical indicators Wap65-1, gene expression |
