| 摘要: |
| 为揭示太湖水体中浮游病毒的分布规律, 了解其在微食物环中的生态功能, 2013年9月至2014年5月间, 采用SYBR Green I荧光显微计数法, 研究了太湖水体中浮游病毒的时空分布特征, 并探讨了影响水体中病毒丰度的主要环境因子。结果表明: (1) 太湖水体中浮游病毒的丰度具有显著的空间差异, 网围养殖区的病毒丰度较高, 为(3.52±0.28)×107 ind./mL, 敞水区的病毒丰度较低, 为(4.06±0.90)×106 ind./mL;(2) 浮游病毒的丰度也具有显著的时间差异, 秋季浮游病毒的丰度为(2.83±0.74)×107ind./mL, 显著大于春季(3.11±0.67)×106ind./mL;(3) 太湖水体中浮游病毒的丰度与叶绿素a浓度之间具有显著的正相关性, 但与异养细菌丰度间的相关性不显著, 表明太湖水体浮游病毒以浮游藻类病毒为主。 |
| 关键词: 太湖 浮游病毒 荧光显微镜 叶绿素a 病毒-细菌比 |
| DOI：10.11693/hyhz20150300071 |
| 分类号: |
| 基金项目:江苏省农业科技自主创新资助项目,cx(13)3049号。 |
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| SPATIAL-TEMPORAL DISTRIBUTION OF VIRIOPLANKTON AND THEIR RELATIONSHIP WITH ENVIRONMENTAL FACTORS IN TAIHU LAKE |
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DUAN Cui-Lan1, HU Zhi-Xin2, ZOU Yong1, CHEN Guang-Yun1, CHEN Jing1, WANG Yi-Ming2, ZHANG Yong-Jiang1
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1.Monitoring Center for Fishery Ecologic Environment of Jiangsu Province, Nanjing 210036, China;2.School of Environmental Engineering, Nanjing Institute of Technology, Nanjing 211167, China
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| Abstract: |
| We studied the spatiotemporal distribution of virioplankton and the relationship among virioplankton, bacterioplankton and Chl a in Taihu Lake, Jiangsu, East China, based on a large-scale survey conducted in Sep.2013 and May.2014.Virioplankton was stained with SYBR GreenⅠand analyzed in epifluorescence microscopy.The virioplankton abundance had significant spatial difference.The highest abundance (3.52±0.28)×107 ind./mL occurred in the enclosure culture area, while the lowest (4.06±0.90)×106 ind./mL in the open water.The virioplankton abundance had also significant temporal difference.It was greater in autumn than that in spring.Moreover, virioplankton abundance was more closely correlated to Chl a concentration (P<0.05) than to bacterial density (P>0.05), indicating that phytoplankton virus was the dominant species of virioplankton in Taihu Lake. |
| Key words: Taihu Lake viroplankton epifluorescence microscopy chlorophyll a VBR |
