| 摘要: |
| 分别采用磷酸二氢钾(KH2PO4)、β-甘油磷酸钠(Sodium-β-glycerophosphate, β-G-P)、葡萄糖-6-磷酸(Glucose-6-phosphate, G-6-P)、卵磷脂(Lecithin, LEC)和三磷酸腺苷二钠(Adenosine disodium triphosphate, ATP)作为f/2培养基的磷源,探讨了不同形态磷源对具槽帕拉藻(Paralia sulcata)生长及磷酸酶活性的影响。结果发现,具槽帕拉藻能够有效利用上述五种磷源,且溶解有机磷(Dissolved organic phosphorus, DOP)比KH2PO4更有利于具槽帕拉藻的生长;对碱性磷酸酶(Alkaline phosphatase, AP)活性分析发现,大分子DOP(LEC)可能需要通过磷酸酶的水解作用才能被吸收利用。在胞外磷充足的情况下,具槽帕拉藻能够在胞内储存磷源,而在磷限制时则可利用胞内储存的磷源来维持藻细胞的生长。 |
| 关键词: 具槽帕拉藻(Paralia sulcata) 溶解有机磷 碱性磷酸酶 酸性磷酸酶 |
| DOI：10.11693/hyhz20141100315 |
| 分类号: |
| 基金项目:中国科学院战略性先导科技专项(A类)项目,XDA11020405号。 |
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| EFFECTS OF DIFFERENT PHOSPHORUS SUBSTRATES ON GROWTH AND PHOSPHATASE ACTIVITY OF ALGAE PARALIS SULCATA |
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YU Qian1,2, WANG Qing1, YUAN Ze-Yi3, WU Hui-Feng1, ZHAO Jian-Min1
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1.Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.National Marine Data and Information Service, Tianjin 300171, China
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| Abstract: |
| We used five phosphorus substrates, i.e., KH2PO4, sodium-β-glycerophosphate(β-G-P), glucose-6-phosphate(G-6-P), lecithin(LEC), and adenosine disodium triphosphate(ATP) to explore the effects on growth and phosphatase activity of Paralia sulcata. It was found that P. sulcata could utilize all five phosphorus substrates to support the growth, and DOP(dissolved organic phosphorus) was better than orthophosphate for the growth of P. sulcata. The change in alkaline phosphatase(AP) activity suggested that large molecular DOP(LEC for example) could be utilized in phosphatase hydrolysis. Furthermore, high external phosphorus concentration would eventually cause high intracellular phosphorus in P. sulcata. Therefore, P. sulcata could utilize intracellular phosphorus to sustain growth under depletion of dissolved phosphorus. |
| Key words: Paralia sulcata dissolved organic phosphorus alkaline phosphatase intracellular phosphorus |
