| 摘要: |
| 为提高一株海洋解淀粉芽孢杆菌(Bacillus amyloliquefaciens)ESB-2生产大环内酯类抗生素Macrolactin A的能力,利用大孔树脂对发酵液进行粗提取,利用高速逆流色谱法,高效、快速分离制备出高纯度的Macrolactin A。采用高效液相色谱法检测不同碳源对Macrolactin A产量的影响,且在优化基础上采用摇瓶发酵得到海洋解淀粉芽孢杆菌的发酵过程曲线。结果表明:高速逆流色谱法能够高效快速的制备出Macrolactin A纯品,纯度达到95%以上,明显优于高效液相色谱;添加葡萄糖、蔗糖和麦芽糖均有利于Macrolactin A的生成,麦芽糖为最佳碳源,最佳浓度为1%,其产量达到18.5mg/L,是优化前的2倍多。本研究可为提高Macrolactin A的产量、解决其药源问题、实现规模化生产打下了良好的基础。 |
| 关键词: 高速逆流色谱 海洋解淀粉芽孢杆菌 Macrolactin A 碳源优化 |
| DOI：10.11693/hyhz20141200363 |
| 分类号: |
| 基金项目:国家自然科学基金项目,41306134号。 |
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| OPTIMIZATION OF CARBON RESOURCE FOR MACROLACTIN A PRODUCTION FROM MARINE BACILLUS AMYLOLIQUEFACIEN |
|
HOU Dong-Yuan, WU Zu-Fang, ZHANG Xin
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Ningbo University, Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo 315211, China
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| Abstract: |
| To optimize the production of Macrolactin A from a marine Bacillus amyloliquefaciens ESB-2, we extract crude material with macroporous adsorptive resin and then purified in high-speed counter-current chromatography(HSCCC). The purity of separated component was determined and the influence of different carbon sources on Macrolactin A yield was detected, in high performance liquid chromatography(HPLC). The fermentation curve of marine Bacillus amyloliquefaciens was obtained under optimization condition. The results show that Macrolactin A could be purified quickly in HSCCC, and the purity of the product reached >95%. The carbon sources of glucose, sucrose, and maltose were conducive for the accumulation of Macrolactin A, and maltose was the best carbon source at concentration of 1%, under which the concentration of Macrolactin A could reach 18.5 mg/L and the production was more than doubled. |
| Key words: high-speed counter-current chromatography(HSCCC) Bacillus amyloliquefaciens Macrolactin A carbon resource optimization |
