| 摘要: |
| 为建立一种简单快捷的水产品中多氯联苯(PCBs)检测方法,本研究应用电子鼻对青蛤(Cyclina sinensis)软组织中富集的多氯联苯进行检测,并采用HS-SPME-GC-MS(顶空固相微萃取-气质联用)对结果进行了验证。首先利用电子鼻制作PCB15浓度梯度模版,检测PCB15处理72h以及144h的青蛤软组织中残留量,采用LDA和PCA两种方法统计并同模板的浓度进行比较。结论:青蛤软组织中PCB15浓度能明显分开,并随着处理时间的延长,区分效果愈加明显,区分度愈大。PCA的分辨效果明显优于LDA。运用欧氏距离、马氏距离、判别函数法和相关性对含有PCB15的样品浓度分别进行鉴别和验证,四种方法总的鉴别率均达到90%以上,能够较准确地对样品进行验证。电子鼻检测青蛤软组织中的多氯联苯的最低检测限为0.05μg/L, GC-MS为0.5μg/L。 |
| 关键词: 多氯联苯 青蛤软组织 电子鼻 顶空固相微萃取-气质联用 |
| DOI：10.11693/hyhz20150700183 |
| 分类号: |
| 基金项目:海洋公益性行业科研专项经费资助项目,201005016号。 |
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| STUDY OF FAST DETECTION OF PCBs IN CYCLINA SINENSIS(GMELIN) BASED ON ELECTRONIC NOSE |
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LV Yan1, WANG Zu-Zhong1, HU Ling-Ping1, HUANG Jian2, ZHOU Jun1, ZHANG Chun-Dan1, LI Ye1, SU Xiu-Rong1
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1.School of Marine Science, Ningbo University, Ningbo 315211, China;2.Beijing Purkinje General Instrument Co., Ltd., Beijing 101200, China
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| Abstract: |
| To establish a simple and quick detection technology of PCBs in seafood, polychlorinated biphenyls(PCBs) in Cyclina sinensis(Gmelin) was detected by the electronic nose, and the results were verified by HS-SPME-GC-MS(headspace solid-phase microextraction-gas chromatography-mass spectrometer). The results showed that C. sinensis in different concentrations PCB15 within 72h, and 144h could be identified by electronic nose using LDA and PCA analysis. The seawater could also be identified by electronic nose. At the same time, with the extension of treatment time, discrimination became more obviously, the greater the discrimination, the greater contribution rate. Euclidean distance, Mahalanobis distance, discriminant function method and correlation were also used on sample identification with different concentrations of PCB15. The total rate of four methods were all more than 80%, and the samples could be accurately verified. The results of HS-SPME-GC-MS detection showed that the lowest detection limit of PCBs concentration reached to 0.5μg/L, and the estimated value is 0.05μg/L which was detected by electronic nose. |
| Key words: PCBs soft tissue of Cyclina sinensis electronic nose HS-SPME-GC-MS |
