| 摘要: |
| 为提高鱿鱼加工下脚料的利用率,以秘鲁鱿鱼肝脏为研究对象,采用生物酶解技术和凝胶蛋白分离技术等对其进行研究。研究结果表明:鱿鱼肝脏蛋白酶解液经过超滤处理后(分子质量为20 kDa),用Sephadex G-100初步分离,得到五个组分,对分离的组分进行研究,结果表明降血压活性能力最强的是组分二,测定其半抑制浓度(IC50)为1.8mg/mL。对组分二进行活性条件分析,结构表明其ACE抑制活性在温度0-100℃、pH 1-12之间基本没有变化。最后用DEAE阴离子交换柱和Sephadex LH-20对组分二继续分离纯化,得到组分六和组分七,测定其半抑制浓度(IC50)分别达到1.52 mg/mL和2.16 mg/mL。本研究可为利用鱿鱼加工下脚料开发降血压活性肽产品提供理论基础。 |
| 关键词: 鱿鱼肝脏 ACE活性抑制肽 分离纯化 生物活性分析 |
| DOI：10.11693/hyhz20150600174 |
| 分类号: |
| 基金项目:浙江省自然科学基金项目,LY13C00005号,LQ15C200009号;舟山市科技计划项目,2014C41011号,2014C31051号;浙江海洋学院科研启动资助项目,Q1424号。 |
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| ACE INHIBITORY PEPTIDE IN SQUID LIVER HYDROLYSATE:SEPARATION, PURIFICATION, AND BIOACTIVITY ANALYSIS |
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LIANG Jia, XIE Chao, LIN Lin, HUANG Ju, WANG Ting, YU Qun-Di
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Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, College of Food and Medicine, Zhejiang Ocean University, Zhoushan 316022, China
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| Abstract: |
| For make use of leftover from squid industry, we separated and purified the inhibiting peptide from squid liver and studied its bioactivity by hydrolyzing squid liver protein. Enzymolysis and gel filtration separation technology were applied. Results show that after the enzyme solution was ultra-filtrated(molecular weight 20kDa), five components were separated on Sephadex G-100. Among them, Component 2 displayed a high ACE(angiotensin converting enzyme)-inhibitory activity with half-inhibitory concentration(IC50) at 1.92mg/mL. In addition, the ACE inhibitory activity remained largely no change in temperature of 0-100℃ and 1-12 in pH. Further purification on Component 2 in DEAE anion exchange column on Sephadex LH-25, additional two components 8 and 9 in IC50 of 1.58 and 2.12 mg/mL, respectively. Therefore, the result may offer a reference to research and develop medicines for high-blood pressure treatment. |
| Key words: squid liver waste ACE inhibitory peptide separation and purification bioactivity analysis |
