| 摘要: |
| 采用Illumina MiSeq高通量测序技术,对杭州西溪湿地4种不同植被下沉积物的细菌多样性进行了研究。通过提取样品基因组DNA,对16S rRNA V3、V4区测序,共获得有效序列67734条,产生2181个OTU,序列平均长度为441 bp,其中大于400bp的序列占99.74%。对97%相似水平的OTU代表序列进行分类学分析,结果表明,沉积物具有很高的细菌多样性,涵盖了30个门252个属的细菌。其中变形菌门(Proteobacteria)是各样品的优势类群,所占比例高达30.0%-64.7%。同时, β-变形菌纲、γ-变形菌纲和δ-变形菌纲在变形菌门中占主导地位,在这些类群中发现有大量与N、S等元素代谢相关的菌群,应该在湿地沉积物的元素循环中发挥着重要作用。此外, 4个样品中约有10%-15%的序列属于无法确定分类位置(Unclassified)的类群,说明西溪湿地沉积物中蕴藏有较多的潜在新物种。本研究结果将为西溪湿地的保护和修复提供理论和实践依据。 |
| 关键词: 西溪湿地 沉积物 细菌多样性 Illumina MiSeq高通量测序 |
| DOI：10.11693/hyhz20150700192 |
| 分类号: |
| 基金项目:浙江省自然科学基金项目,LY14C010004号;浙江省科技计划项目,2014C33061号。 |
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| BACTERIAL DIVERSITY AND COMMUNITY STRUCTURE IN SEDIMENTS OF XIXI WETLAND, HANGZHOU |
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HUANG Yuan1, FANG Xu2, CHU Wen-Ke1, CHEN Min1
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1.College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China;2.Zhejiang Institute of Microbiology, Hangzhou 310012, China
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| Abstract: |
| The Illumina MiSeq sequencing-based analysis of the V3-V416S rRNA gene region was used to detect bacterial diversity and community structure in Xixi wetland, Hangzhou. Overall, 67734 optimized reads with an average length of 441bp were obtained from four sediment samples. Bacterial diversity was very high in these samples, since a total of 2181 OTUs were identified which could be assigned into 30 different phyla and 252 genera. The dominant group belonged to Proteobacteria, accounting for 31.0%-64.7% of total sequences. Among Proteobacteria, the beta-, delta-and gamma-subdivisions were dominant classes. Chloroflexi, Firmicutes and Bacteroidetes were also very common, accounting for 7.0%-43.0%, 1.1%-14.7% and 1.0%-10.2% of total libraries, respectively. The ordination analysis showed that there were about 10%-15% of total sequences belonged to the unclassified group, which meant that there may be many undiscovered species in sediments. There were also large number of DNA sequences affiliated to the strains which related to N and S metabolism in beta-, gamma-, and delta-Proteobacteria branches. This study will lay the foundation for future research on microbiology in Xixi wetland. |
| Key words: Xixi wetland sediment bacterial diversity MiSeq high-throughput sequencing |
