| 摘要: |
| 应用分子生态学方法, 研究在不同芳香族化合物胁迫下海洋沉积物中细菌群落结构的演替 规律。在实验室建立驯化系统, 在海洋沉积物中分别添加芘和苯甲酸钠, 采用Illumina MiSeq 高通量 测序技术分析实验组和对照组细菌群落16S rRNA 基因V3-V4 可变区数量的组成及变化, 研究沉积物 中添加芘、苯甲酸钠后, 微生物群落多样性和群落结构的变化。实验结果表明: 芘、苯甲酸钠极大地 降低了海洋沉积物中菌群丰度和多样性; 在添加苯甲酸钠的实验组, 变形杆菌门中海杆菌属 (Marinobacter)、假海源菌属(Pseudidiomarina)的细菌显著富集; 在添加芘实验组中拟杆菌门中的 Balneola 属、碳酸噬胞菌属(Aequorivita)极大富集。初步分析了在海洋沉积物环境中不同多环芳烃胁迫 下细菌群落结构的演替特征, 实验结果为海洋环境中针对不同芳香烃的微生物修复提供理论依据。 |
| 关键词: 海洋细菌 群落结构 多样性 苯甲酸钠 芘 |
| DOI：10.11693/hyhz20141200345 |
| 分类号: |
| 基金项目:中国科学院战略性先导科技专项项目, XDA11020403 号; 国家自然基金面上项目, 41376138 号。 |
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| RESPONSE OF BACTERIAL COMMUNITIES TO PYRENE AND BENZOATE SODIUM IN MARINE SEDIMENTS |
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SUN Xiang-Nan1,2, HU Xiao-Ke1, WANG Hui1
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1.Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;2.University of Chinese Academy of Sciences, Beijing 100049, China
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| Abstract: |
| We set up microcosm experiments to study dynamic changes in the bacterial communities, and in which we supplemented with different model PAH compounds pyrene and sodium benzoate. High-throughput sequencing technology Illumina Miseq targeting the 16S rRNA gene V2-V3 variable region was used to analyze changes in composition of the bacterial communities after treated with pyrene or sodium benzoate. Results show that either pyrene or sodium benzoate could dramatically affect the bacterial community structure in marine sediments, and largely reduced the diversity and abundance. Marinobacter and Pseudidiomarina were boosted in sodium-benzoate-enriched group, while Balneola and Aequorivita were greatly enriched in pyrene-adding group. The results may help understanding some functional bacteria capable of degrading pyrene and sodium benzoate, and providing a theoretical basis for bioremediation of PAH compounds in marine environment. |
| Key words: bacterial community diversity sodium benzoate pyrene dynamic changes |
