| 摘要: |
| 为研究椒江化工园区邻近海域沉积物中细菌结构, 选取四个典型站点, 通过分离纯化、鉴 定和建立克隆文库的方法, 对其进行多样性及系统发育分析。可培养细菌的形态学及API 鉴定结果 显示洋葱假单胞菌及杀鲑气单胞菌杀鲑亚种是优势种, 典型细菌16S rDNA 分子鉴定结果表明厚壁 菌门及γ-变形菌纲为主要类群。非培养细菌克隆文库的序列分析结果表明: 细菌主要包括变形菌门、 酸杆菌门、绿弯菌门、芽单胞菌门、硝化螺旋菌门、CFB 群、放线菌门、厚壁菌门、浮霉菌门等9 个类群; 其中C3 站点克隆子主要属于γ-变形菌纲及δ-变形菌纲; C4 站点克隆子以γ-变形菌纲及酸杆 菌门为主; C5 站点克隆子主要属于δ-变形菌纲及γ-变形菌纲; C6 站点克隆子主要属于γ-变形菌纲及 放线菌门。综合可培养及非培养结果, 可发现椒江口化工园区邻近海域沉积物中γ-变形菌纲为典型 优势类群, 除相当数量的克隆子相似序列来自石油烃污染的沉积环境外, 还有大量克隆子相似序列 来自养殖污染环境。 |
| 关键词: 变形菌 API 16S rDNA 群落结构 |
| DOI：10.11693/hyhz20150900246 |
| 分类号: |
| 基金项目:国家自然科学基金面上项目, 31270160 号; 浙江省自然科学基金项目, LY12C03003 号; 浙江省环保厅科研项目, 2012A033 号, 2013A020 号。 |
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| BACTERIAL DIVERSITY IN SEDIMENTS IN JIAOJIANG CHEMICAL INDUSTRIAL PARK ADJACENT AREA |
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JIANG Ran1, WANG Jian-Xin1, HUANG Bei2, ZHANG Pan1, ZHENG Jun-Wei1, YU Kai-Cheng1, LIU Ming-Hua1
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1.Zhejiang Ocean University, Laboratory for Marine Microbial Molecular Ecology and Application, Zhoushan 316022, China;2.Zhoushan Marine Ecological and Environmental Monitoring Station in Zhejiang Province, Zhoushan 316021, China
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| Abstract: |
| We studied the community structure of bacteria in sediment from four sites in Jiaojiang chemical industrial park adjacent area and phylogenesis in bacterial clone library by isolation, purification, and identification procedures. The results of 16S rDNA analysis showed that for cultured bacteria, Aeromonas salmonicida was the main species, and Firmicutes and γ-Proteobacteria were the dominant groups; while non-cultured bacteria included mainly Proteobacteria, Acidobacteria, Chloroflexi, Gemmatimonadetes, Nitrospira, CFB group, Actinobacteria, Firmicutes and Planctomycetes. Each site featured own bacterial clone. In overall, γ-Proteobacteriain was the dominant group in sediment of Jiaojiang chemical industrial park adjacent area, and the similar sequences of many typical clones were probably derived from environment pollution by petroleum hydrocarbon, aquaculture and heavy metals. |
| Key words: Proteobacteria API 16S rDNA community structure |
