| 摘要: |
| 应用荧光定量PCR技术, 检测了TLR21 基因在牙鲆(Paralichthys olivaceus)感染迟缓爱德华 氏菌(Edwardsiella tarda)后, 在0 h、1 h、3 h、6 h、12 h、1 d、3 d、6 d 后, 在心脏、肝脏、脾脏、 头肾、鳃、小肠、肌肉和血的时空表达特征, 并探讨了它们与牙鲆先天免疫反应的关系。结果表明, 大 多组织在感染病原6 h 后TLR21 基因表达明显上调, 尤其是头肾和小肠。头肾6 h 的表达量达到了 对照组的59.3 倍, 小肠6 h 的表达量为对照组的38.6 倍。迟缓爱德华氏菌感染引起牙鲆体内各组织 中TLR21 的上调表达和变化, 为研究牙鲆对迟缓爱德华氏菌的防御机制提供了理论依据。 |
| 关键词: TLR21 基因 牙鲆 迟缓爱德华氏菌 表达 实时荧光PCR |
| DOI：10.11693/hyhz20150800211 |
| 分类号: |
| 基金项目:天津市自然科学基金重点项目资助, 14JCZDJC34200 号; 天津市教育科学十二五规划课题, BE4142 号。 |
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| EXPRESSION OF TLR21 GENE IN PARALICHTHYS OLIVACEUS CHALLENGED BY EDWARDSIELLA TARDA |
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ZHANG Jie1, ZHENG Jin-Hui1, LI Qing-Ya1, GENG Xu-Yun2, SUN Jin-Sheng1,2, PAN Bao-Ping1, SUN Shi-Nan1, GAO Hong1
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1.Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, China;2.Tianjin Aquaculture Disease Prevention & Treatment Center, Tianjin 300221, China
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| Abstract: |
| Expression patterns of Paralichthys olivaceus TLR21 gene in the heart, liver, spleen, head kidney, grill, intestine, muscle, and blood were characterized in quantitative real-time PCR at 0h, 1h, 3h, 6h, 12h, 1d, 3d and 6d after infection by Edwardsiella tarda. The results show that E. tarda induced TLR21 that regulates the relative expression levels in P. olivaceus tissues, and the expression changes were tissue specific. The expression was up-regulated in all the tissues and increased by 59.3-fold and 38.6-fold in spleen and intestine respectively in 6 h after challenge. Therefore, TLR21 gene in P. olivaceus is vital for the physiological function of the fish. This finding offers a theoretical reference for understanding the mechanism of disease defense in the fish, with which more effective vaccines and immune therapies may be developed. |
| Key words: TLR21 Paralichthys olivaceus Edwardsiella tarda expression real-time PCR |
