| 摘要: |
| 本文探讨牛磺酸在鲫鱼生长、肠道细胞增殖分化以及蛋白质消化吸收相关基因的表达等方 面的作用。试验以480 尾[初始体质量(29.07±0.19)g]鲫鱼(Carassius auratus)为研究对象, 随机分成6 组, 每组设定4 个重复, 每个重复有鱼20 尾。鲫鱼基础饲料中添加的牛磺酸的剂量分别是0.0、1.0、 2.5、4.0、6.0 以及9.0 g/kg, 以0.0 g/kg 添加组为对照组, 试验持续8 周。结果表明: (1) 牛磺酸对鲫 鱼有促生长作用, 6.0 g/kg 添加组的鱼的增重率, 特定生长率, 蛋白质效率, 饲料系数等都显著高于 对照组(P<0.05).(2) 牛磺酸能促进肠绒毛的生长, 降低隐窝深度。(3) 牛磺酸提高了鲫鱼血清中T3、 T4 的含量, 添加6.0 g/kg 时显著高于对照组, 且此时血清尿素氮浓度显著低于对照组和其它处理组 (P<0.05).(4) 氨肽酶N(APN)、小肽转运蛋白(PepT1)、基因系尾型同源盒基因(CDX2)、L-氨基酸转 运载体蛋白(LAT2)等基因的相对表达量随牛磺酸剂量增加而上调, 6.0 g/kg 时显著高于对照组和其它 实验组(P<0.05).由此可见, 适量添加牛磺酸能影响鲫鱼生长、肠道细胞增殖及蛋白消化吸收相关基 因的表达。 |
| 关键词: 牛磺酸 鲫鱼 生长 蛋白质 细胞增殖 |
| DOI：10.11693/hyhz20150800227 |
| 分类号: |
| 基金项目:国家自然科学基金项目, 31272675 号; 湖南省教育厅优秀青年项目, 13B144 号; 湖南省高校产业化培育项目, 13CY031 号。 |
|
| PROPER TAURINE-ENRICHED FISHMEAL PROMOTES GROWTH OF CRUCIAN CARP CARASSIUS AURATUS |
|
WU Qin1,2, TANG Jian-Zhou2, LIU Zhen3,2, ZHAO Yu-Rong1, HAO Guang2, LU Shuang-Qing2
|
|
1.College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;2.Department of Biotechnology & Environment Engineering, Changsha University, Changsha 410003, China;3.Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde 415000, China
|
| Abstract: |
| To study the effect of taurine-enriched feed on growth of Carassius auratus, taurine in different concentrations (0.0, 1.0, 2.5, 4.0, 6.0, and 9.0 g/kg) was tested in 8 weeks, during which proliferation of intestinal cell and expressions of genes associated with protein digestion and absorption were studied. A total of 480 individuals in body weight of 29.07±0.19g were selected and randomly divided into 6 treatments containing 4 replicates each with 20 individuals. The basal diet with no taurine was used as the control. The results show that taurine could promote the growth performance best at 6.0 g/kg, at which the weight gain rate, specific growth rate, feed coefficient, and protein efficiency ratio increased remarkably than the control (P>0.05). In specific, taurine-addition promoted the growth of intestinal villus, reduced crypt depth remarkably, enhanced the serum T3 and T4 concentrations, and reduced serum urea nitrogen concentration significantly (P>0.05). In addition, taurine enhanced remarkably the relative expression of APN, PepT1, LAT2, and CDX2 mRNA. Therefore, a proper amount of taurine supplementation is necessary for the proliferation of intestinal cell, genes associated with protein digestion and absorption of the fish, favoring the growth performance. |
| Key words: taurine crucian carp growth protein cellular proliferation |
