| 摘要: |
| 从长江口及邻近海域10 个站点采集表层海水, 通过DAPI 染色计数和克隆文库构建的方法, 对细菌的多样性及群落结构进行初步研究。DAPI 染色计数结果显示长江口及邻近海域表层海水中 细菌丰度总体较高, 生物量变化较大, 从1.16×105(A04 站点)到 1.48×106 cells/mL (B03 站点); 总体 趋势从长江口向外海增加。细菌克隆文库的系统发育分析表明: 细菌序列以变形菌门为主(占总文库 的60.61%), 其中α-变形菌纲是绝对优势类群(50.62%); 其次是拟杆菌门(15.18%)、放线菌门(14.79%) 和蓝细菌门(4.61%), 还有少量厚壁菌门、绿弯菌门、酸杆菌门、浮霉菌门、纤维杆菌门、疣微菌门 等; 另外柔膜菌门和SAR 类群也有发现。文库多样性分析结果显示长江口及邻近海域部分站点表层 海水中细菌多样性显著, 与研究区海域水产养殖活动有关, 并受到区域洋流和海洋化学环境(如低氧 带)的影响。 |
| 关键词: 长江口 细菌 多样性 DAPI 16S rDNA |
| DOI：10.11693/hyhz20150900243 |
| 分类号: |
| 基金项目:国家自然科学基金项目, 31270160 号, J1310037 号; 浙江省自然科学基金项目, LY12C03003 号。 |
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| BACTERIAL DIVERSITY AND COMMUNITY STRUCTURE IN SURFACE SEAWATER OF CHANGJIANG RIVER ESTUARY AND ADJACENT AREAS |
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LIU Xiao-Hui, WANG Jian-Xin, WANG Shuai-Bing, FAN Ying-Ping, YU Kai-Cheng, JIANG Ran, LIU Ming-Hua
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Zhejiang Ocean University, Laboratory for Marine Microbial Molecular Ecology and Application, Zhoushan 316022, China
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| Abstract: |
| We sampled surface seawater in 10 stations in Changjiang River estuary and adjacent areas, and studied the diversity and community structure of bacteria in seawater, showing high abundance of bacteria in the study region ranging between 1.16×105 (Station A04) and 1.48×106 cells/mL (B03) by DAPI direct count. Among them, 60.61% the total clone library was Proteobacteria, of which Alphaproteobacteria took 50.62%, Bacteroidetes (15.18%), Actinobacteria (14.79%), Cyanobacteria (4.61%), few Firmicutes, Chloroflexi, Acidobacteria, Planctomycetes, Fibrobacteres, and Verrucomicrobia, and occasionally Tenericutes and SAR groups as shown in phylogenetic analysis on the clone libraries. Therefore, the bacteria in the surface water of Changjiang River estuary and adjacent areas was abundant and diversified in complicated structures, reflecting the impacts of local aquaculture activities and regional marine physical-chemical settings, for example, the oxygen depletion at sea bottom and Changjiang River water dilution. |
| Key words: Changjiang River estuary bacteria diversity DAPI 16S rDNA |
