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长牡蛎(Crassostrea gigas)中ChIP-seq 方法的建立
展望, 王文, 施威扬
同济大学生命科学与技术学院 上海 200092
摘要:
染色质免疫共沉淀技术(ChIP)作为研究DNA 结合蛋白与DNA 相互作用最有力的工具, 越 来越受到重视。但目前该技术在牡蛎等贝类中的应用尚未见相关报道。为了研究长牡蛎早期胚胎发 育过程中表观遗传信息的变化模式, 本文在长牡蛎胚胎中探索并建立了一套完整的染色质免疫共沉 淀方法, 构建了高质量的高通量测序文库, 首次获得了牡蛎全基因组水平的组蛋白H3K4me3 修饰分 布, 从而为研究牡蛎的表观遗传调控奠定了基础。
关键词:  长牡蛎(Crassostrea gigas)  ChIP-seq  高通量测序  组蛋白修饰
DOI：10.11693/hyhz20150500141
分类号:
基金项目:上海市浦江人才计划项目, 14PJ1408400 号; 同济大学985 启动项目, 2000141505 号。
DEVELOPMENT OF A ChIP-seq METHOD IN CRASSOSTREA GIGAS
ZHAN Wang, WANG Wen, SHI Wei-Yang
School of Life Sciences and Technology, Tongji University, Shanghai 200092, China
Abstract:
Chromatin immunoprecipitation coupled with high throughput sequencing (ChIP-seq) is a powerful tool to analyze DNA-protein interactions. However, the application of ChIP-seq in Crassostrea gigas has not been investigated. In this study, we established a protocol for performing ChIP-seq experiment in oyster early embryos. We successfully performed H3K4me3 ChIP-seq on 16-cell stage oyster embryo and revealed the genome-wide distribution of the H3K4me3 histone marks. This study is the first step toward understanding the epigenetic regulation of oyster embryogenesis.
Key words:  Crassostrea gigas  ChIP-seq  high-throughput sequencing  histone modification
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