| 摘要: |
| 近年来,"绿色"生物合成纳米抑菌材料以其方法简单、价格低廉、环境友好的优势已成为纳米技术领域中的一项激动人心的发展策略。为了研究生物合成纳米银(Silver nanoparticles,AgNPs)对水产病原菌的抑菌效果,本研究分别以山楂果实超纯水提取物和山楂乙醇提取物作为还原剂和稳定剂,采用生物合成法制备出两种纳米银抑菌材料W-AgNPs和E-AgNPs。通过紫外-可见吸收光谱(UV-vis)、透射电镜(TEM)和X射线衍射(XRD)等方法对合成产物的光学特性、形貌特征以及晶体结构等指标进行了比较,并采用琼脂扩散法初步检测了W-AgNPs和E-AgNPs对大肠杆菌和金黄色葡萄球菌两种典型病原菌的抑菌活性。研究结果显示,W-AgNPs的合成效果较为理想,它的粒径较小、更加均一,并且具有较高的抑菌活性。在此基础上,我们进一步检测了W-AgNPs对鳗弧菌、溶藻弧菌、副溶血弧菌及点状气单胞菌等4种典型的水产病原菌的抑菌圈,并测定了其抑菌动力学曲线、最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。研究结果表明,W-AgNPs对四种供试水产病原菌均有显著的抑菌效果,10μg/mL和20μg/mL的W-AgNPs均对鳗弧菌的生长产生明显的抑制作用,对鳗弧菌的MIC和MBC分别为6.5μg/mL和12.9μg/mL。研究表明山楂提取物制备的纳米银抑菌剂对水产病原菌有显著的抑制和杀灭效果,在水产养殖领域中有着广阔的开发和利用前景。 |
| 关键词: 山楂 纳米银 生物合成 水产病原菌 抑菌活性 |
| DOI：10.11693/hyhz20150900248 |
| 分类号: |
| 基金项目:国家自然科学基金面上项目,31570649号;山东省自然科学基金面上项目,ZR2013CM018号;山东省高校科研计划项目,J15LE03号。 |
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| BIOSYNTHEZIED SILVER NANOPATICLES USING HAWTHORN FRUIT EXTRACT AND THEIR ANTIBACTERIAL ACTIVITY AGAINST FOUR COMMON AQUATIC PATHOGENS |
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JIANG Yu,LIU Chong-Chong,LI Fu-Yan,XU Hui,SUN Hu-Shan and WANGLei
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College of Life Sciences,Ludong University,College of Chemistry and Material Sciences,Ludong University,College of Life Sciences,Ludong University,College of Chemistry and Material Sciences,Ludong University,College of Life Sciences,Ludong University,College of Life Sciences,Ludong University
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| Abstract: |
| Green synthetization of nano-antibacterial materials has become a promising strategy in nanotechnology in the past few years because of its simple, low-cost, and eco-friendly advantages. To study the antibacterial effect of biosynthesized silver nanoparticles (AgNPs) against aquatic pathogenic bacteria, we synthesized AgNPs using water and ethanol extracts of hawthorn fruit as reducing and stabilizing agent, and named them as W-AgNPs and E-AgNPs, respectively. In addition, we studied the formation of W-AgNPs and E-AgNPs in the surface plasmon resonance band illustrated by UV-vis spectrophotometer, observed their morphology in transmission electron microscopy, and investigated the crystalline structure of them in X-ray diffraction. Moreover, we conducted a preliminary antimicrobial study on W-AgNPs and E-AgNPs against two common pathogens: Escherichia coli and Staphylococcus aureus. The results showed that W-AgNPs was smaller and more homogeneous in size, moreover, it had higher antibacterial activity. Further antibacterial study showed that W-AgNPs possessed remarkable antibacterial activity against all aquatic pathogens tested, including Vibrio anguillarum, Vibrio anguillarum, Vibrio parahaemolyticus, and Aeromonas punctata. As shown in bacteriostasis kinetic curves, W-AgNP concentrations at 10 and 20 μg/mL could significantly inhibit the growth of V. anguillarum. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of W-AgNPs against V. anguillarum were 6.5 and 12.9 μg/mL, respectively. Therefore, biosynthesized AgNPs using water extract of hawthorn fruit can be used as an effective and broad-spectrum antibacterial agent in promising potential for aquaculture. |
| Key words: hawthorn silver nanoparticles biosynthesis aquatic pathogens antibacterial activity |
