| 摘要: |
| 为研究不同类型Toll样受体基因在凡纳滨对虾免疫调控机制中的作用, 实验首先通过荧光定量PCR方法检测了凡纳滨对虾3种Toll样受体基因在不同组织中的表达情况。结果表明凡纳滨对虾3种Toll样受体基因mRNA在各组织中均有表达。其中, Toll1基因在肌肉, 血淋巴, 心脏和鳃均有较高表达, 心脏表达量最高; Toll2基因在血淋巴, 心脏和鳃均有较高表达, 其中在鳃表达量最高; Toll3基因在鳃和心脏有较高表达, 其中在鳃表达量最高。实验还对人工感染溶藻弧菌及白斑综合症病毒后凡纳滨对虾3种Toll样受体基因在不同免疫组织的表达变化差异进行研究。感染白斑综合症病毒后凡纳滨对虾血淋巴及鳃中3种Toll样受体基因表达量均显著提高, 并在各自达到表达峰值后逐渐下降直至恢复到感染前水平。感染溶藻弧菌后凡纳滨对虾血淋巴中3种Toll样受体基因表达量均显著提高; 鳃中Toll1及Toll3表达量分别于感染后3h和12h有显著提高, 而Toll2表达量无显著提高。由此推测3种Toll样受体基因均可能参与白斑综合症病毒感染所引起的免疫调控; 而在溶藻弧菌感染所引起的免疫调控中, 除了Toll1, Toll3参与鳃的免疫调控外, Toll2基因还参与血淋巴中的先天性免疫调控。长期感染白斑综合症病毒或溶藻弧菌后, 凡纳滨对虾3种Toll样受体基因在血淋巴与鳃中的表达量均显著升高, 这可能与虾体在长期感染过程中, 需要不断保持较高免疫水平有关。 |
| 关键词: 凡纳滨对虾 Toll样受体 荧光定量PCR 溶藻弧菌 白斑综合症病毒 |
| DOI：10.11693/hyhz20151000276 |
| 分类号: |
| 基金项目:上海海洋大学科技发展专项基金, A2-0209-15-200056号; 上海市现代农业产业技术体系建设项目, 沪农科产字(2014)第5号; 上海市科委部分地方院校能力建设项目, 13320501900号; 上海市农业基础性研究项目, 沪农科攻字(2014)第7-1-11号。 |
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| THE EXPRESSION OF TOLL-LIKE RECEPTORS IN DIFFERENT TISSUES OF LITOPENAEUS VANNAMEI AFTER CHALLENGES WITH WHITE SPOT SYNDROME VIRUS AND VIBRIO ALGINOLYTICUS |
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LI Jing-Jing1, LI Yun1, LIU Hong1, DAI Xi-Lin1, JIANG Fei2, DING Fu-Jiang2
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1.College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;2.Shencao Special Aquatic Product Development Company, Shanghai 201516, China
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| Abstract: |
| In the present research, the expression of three types of Toll-like receptors mRNAs in different tissues of the white shrimp, Litopenaeus vannamei, were detected with Real-time PCR. The results indicated that Toll1 was expressed in muscle, heart, haemocyte and gill with high level, while the highest expression was in the heart. Toll2 was expressed in heart, haemocyte and gill with high level and the highest expression was in the gill. Meanwhile, the highest expression of Toll3 was detected in gill. All the expressions of three types of Toll enhanced significantly after the White Spot Syndrome Virus (WSSV) challenge. The significant enhancements of three types of Toll mRNAs were observed in haemocyte after the Vibrio alginolyticus challenge and the expressions of Toll1 and Toll2 rose remarkably in gill after 3 hours and 12 hours post-V. alginolyticus challenge. Therefore, these results suggest that all the 3 types of Toll-like receptors would regulate the immunisation against the White Spot Syndrome Virus. During the V. alginolyticus infection, the immunisation in gill would be regulated by Toll1 and Toll3, while Toll2 will participate in the regulation of immunisation in haemocyte. Additionally, the expression of 3 types of Toll-like receptors significantly enhanced after 2 weeks post WSSV or Vibrio alginolyticus challenges, which might be involved in the high level of immunisation during the long-term infection. |
| Key words: Litopenaeus vannamei Toll-like receptor real-time PCR Vibrio alginolyticus White Spot Syndrome Virus |
