| 摘要: |
| 由大气中CO2浓度升高引起的海洋酸化，是全球性的重大环境问题之一。本研究采用实验生态学的方法，以塔玛亚历山大藻（Alexandrium tamarense）为研究对象，分析其在CO2加富条件下叶绿素荧光动力学参数及产毒特征的变化。调制叶绿素荧光结果显示，CO2加富对塔玛亚历山大藻的PSⅡ最大光化学量子产量（Fv/Fm）、最大相对电子传递效率（rETRmax）有显著影响（P<0.05），且随着培养时间的增长Fv/Fm、rETRmax均降低，对半饱和光强（Ik）、快速光曲线初始斜率（α）却无显著影响（P>0.05）。结果说明CO2加富能促进塔玛亚历山大藻的PSⅡ最大光化学量子产量，提高其最大光能转换效率和相对最大电子传递效率。高效液相色谱法分析结果显示，该株塔玛亚历山大藻主要产漆沟藻毒素1（GTX1）、漆沟藻毒素4（GTX4）、N-磺酰氨甲酰基毒素（C1）及N-磺酰氨甲酰基毒素（C2）四种PSTs毒素，CO2加富不改变主要麻痹性贝毒（PSTs）的种类组成，但能显著提高氨基甲酸酯类毒素（GTX1、GTX4）产量（P<0.05），而降低N-磺酰氨甲酰基类毒素（C1、C2）产量（P<0.05），说明加富能使塔玛亚历山大藻所产毒素发生转化，进而影响藻细胞的整体毒性。 |
| 关键词: 塔玛亚历山大藻 CO2加富 叶绿素荧光参数 麻痹性贝毒 |
| DOI：10.11693/hyhz20150500145 |
| 分类号: |
| 基金项目:国家海洋局近岸海域生态环境重点实验室开放基金资助项目，201106号；青岛市科技计划基础研究项目，13-1-4-234-jch号；国家自然科学基金青年项目，31101875号；国家海洋局青年海洋科学基金项目，2012123号；国家海洋局第一海洋研究所基本科研业务费专项基金项目，GY02-2011T07号。 |
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| CARBON DIOXIDE ENRICHMENT AFFECTS CHLOROPHYLL FLUORESCENCE AND TOXIN PRODUCTION OF ALEXANDRIUM TAMARENSE |
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XU Jin-Tao1, PANG Min2, MA Xin1, MAO Xue-Wei3, WU Zhen-Xing4, CHEN Hong-Ju3, WANG Yan2, ZHANG Xue-Lei2
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1.Qinhuangdao Marine Environmental Monitoring Central Station of SOA, Qinhuangdao 066002, China;2.Marine Ecology Research Center, First Institute of Oceanography, SOA, Qingdao 266061, China;3.College of Environmental and Engineering, Ocean University of China, Qingdao 266100 China;4.Technique Center of Shandong Exit-Entry Inspection and Quarantine Bureau of China, Qingdao 266002, China
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| Abstract: |
| Ocean acidification, caused by the rising atmospheric CO2, is one of the serious global environmental problems. We investigated the effect of CO2 enrichment on chlorophyll fluorescence and toxin production of Alexandrium tamarense. Results of PAM (Pulse-Amplitude-Modulation) chlorophyll fluorometer show that CO2 enrichment had significant effects on the maximum quantum yield of PSⅡ(Fv/Fm) and the maximum relative electron transport rate (rETRmax) of A. tamarense (P<0.05), and the parameters decreased with the culture time. However, CO2 enrichment had little effects on the minimum saturating irradiance (Ik) or the light energy utilization efficiency (α) (P>0.05). Therefore, the potential maximum quantum yield of PSⅡ might be promoted with enriched CO2 and the optimal light energy conversion efficiency and maximum relative electron transport rate might be improved. In addition, result of toxin detection in high performance liquid chromatography show that A. tamarense mainly produced GTX1, GTX4, C1, and C2 toxins as the main PSTs component. CO2 enrichment did not obviously affect the composition of PSTs toxins, but increased the carbamate toxins (GTX1, GTX4) productions, and reduced N-sulfo-carbamoyl toxins (C1, C2), suggesting that PSTs toxins might be transmuted by enriched CO2. |
| Key words: Alexandrium tamarense CO2 enrichment chlorophyll fluorescence parameters paralytic shellfish poisoning |
