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| 斑点叉尾鮰(Ictalunes punctatus)源中间气单胞菌(Aeromonas media)分离鉴定及药敏特性 |
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杨移斌1,2, 杨秋红1,2, 胥宁1,2, 董靖1,2, 刘永涛1,2, 艾晓辉1,2
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1.中国水产科学研究院长江水产研究所 武汉 430223;2.淡水水产健康养殖湖北省协同创新中心 武汉 430223
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| 摘要: |
| 从湖南常德某养殖场死亡的斑点叉尾（Ictalunes punctatus）肝脏、肾脏、腹水及血水中分离到一株致病性菌株zy02。对该菌进行了形态特征观察、理化特性测定及分子生物学方法鉴定，用PCR方法同时扩增其16S rDNA和gyrB基因，分析了16S rDNA和gyrB两种基因序列的同源性，并构建了系统发生树。经生理生化测定和16S rDNA与gyrB基因序列分析，zy02株为中间气单胞菌。人工感染该菌后发病鱼表现为与自然发病类似症状，且从组织中再分离的细菌特性与原感染菌相同。腹腔注射后该菌株对斑点叉尾的半致死浓度为3.89×106CFU/mL。菌株zy02对多西环素、庆大霉素及左氧氟沙星等高度敏感；对红霉素中度敏感；对阿莫西林、磺胺异噁唑、克林霉素及利福平等5种药物耐药。本研究为斑点叉尾该病防控提供了理论依据。 |
| 关键词: 斑点叉尾(Ictalunes punctatus) 中间气单胞菌(Aeromonas media) 鉴定 药敏特性 |
| DOI：10.11693/hyhz20160500109 |
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| 基金项目:公益性行业（农业）科研专项，201203085号。 |
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| ISOLATION AND IDENTIFICATION OF AEROMONAS MEDIA FROM ICTALUNES PUNCTATUS AND ITS ANTIBIOTIC SENSITIVITY |
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YANG Yi-Bin1,2, YANG Qiu-Hong1,2, XU Ning1,2, DONG Jing1,2, LIU Yong-Tao1,2, AI Xiao-Hui1,2
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1.Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;2.Hubei Freshwater Aquaculture Collaborative Innovation Center, Wuhan 430223, China
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| Abstract: |
| A bacterial strain zy02 was isolated from Ictalunes punctatus suffered from hemorrhage disease in a mass mortality broke out in an aquaculture field in Changde,Hunan Province during Oct.,2015;and it was identified as Aeromonas media by analyzing biochemical reactions and 16S rDNA and gyrB gene sequence.Artificial affected fish by intraperitoneal zy02-injection showed same symptom as that of natural ones with similar morphological and biochemical characteristics.Therefore,A.media was proved to be the pathogen to I.punctatus and the in LC50 was 3.89×106CFU/mL as determined in the Reed-Muench method.Our antibiotic sensitivity test showed that the isolate was very sensitive to doxycycline,gentamicin,levofloxacin,etc.,less sensitive to erythromycin,and not sensitive to amoxicillin,sulfisoxazole,clindamycin,rifampicin etc.This study provides a theoretical basis for the prevention and control of the disease in I.punctatus. |
| Key words: Ictalunes punctatus Aeromonas media identification antibiotic sensitivity |