| 摘要: |
| 香鱼（Plecoglossus altivelis）白细胞衍生趋化因子2（leukocyte cell-derived chemotaxin 2，PaLECT2）可以激活香鱼头肾来源的单核巨噬细胞（monocytes/macrophages，MO/MΦ），提高细菌感染后香鱼存活率。进一步研究显示，PaLECT2与香鱼C型凝集素受体（C-type lectin receptor，PaCLR）相互作用可激活静息状态下香鱼MO/MΦ，增强其免疫活性。在前期基础上，本研究通过抗体封闭实验检测PaCLR是否介导PaLECT2激活LPS刺激的MO/MΦ功能。结果显示，重组PaLECT2成熟肽（rPaLECT2m）显著提高了LPS刺激的香鱼MO/MΦ细胞因子的表达，并增强了其吞噬能力和杀菌活性。封闭PaCLR显著抑制了rPaLECT2m激活LPS刺激的香鱼MO/MΦ细胞因子表达、吞噬和杀菌功能。因此，PaCLR介导PaLECT2可以激活病理状态香鱼MO/MΦ。这一认识进一步揭示了LECT2在病理状态的鱼类巨噬细胞调控和炎症反应中的作用及机制。 |
| 关键词: LECT2 C型凝集素受体 单核巨噬细胞功能 脂多糖刺激 |
| DOI：10.11693/hyhz20161200274 |
| 分类号:Q789;S917 |
| 基金项目:国家自然科学基金项目，31372555号；浙江省自然科学基金项目，LZ13C190001号，LY14C190007号；宁波市自然科学基金项目，2015A610263号；“水产”浙江省重中之重开放基金，xkzsc1417号；浙江省新苗人才计划项目，2015R405011号。 |
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| PACLR MEDIATES THE EFFECT OF PALECT2 ON LPS-STIMULATED MONOCYTES/MACROPHAGES FROM AYU PLECOGLOSSUS ALTIVELIS |
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SHI Yu-Hong, MA Hai-Ling, LIANG Ya-Fang, CHEN Hang, CHEN Jiong
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Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China
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| Abstract: |
| Leukocyte cell-derived chemotaxin 2 of ayu Plecoglossus altivelis (PaLECT2) is shown to activate ayu head kidney-derived monocytes/macrophages (MO/MΦ) in vitro to improve the cure of bacterial-infected fish. Our earlier research reveals that PaLECT2 could activate the resting MO/MΦ and enhance its immuno-activity by interacting with a C-type lectin receptor of ayu (PaCLR), based on which we examined the effect of PaCLR on PaLECT2 activation of LPS-stimulated ayu MO/MΦ using antibody neutralization method. Results show that the recombinant PaLECT2 mature peptide (rPaLECT2m) increased the cytokine expression, and enhanced phagocytosis and bactericidal activity of LPS-stimulated ayu MO/MΦ. By blocking the ectodomain of PaCLR by anti-PaCLR, these effects were inhibited significantly. Therefore, PaCLR could mediate the activation effect of PaLECT2 on LPS stimulated MO/MΦ. This finding revealed the mechanism of LECT2 involvement in the regulation and inflammatory response of the fish monocyte/macrophage. |
| Key words: LECT2 C-type lectin receptor monocyte/macrophage functions LPS stimulation |
