| 摘要: |
| 为了进一步评估由白芍（Radix paeoniae Alba）、杜仲（Eucommia ulmoides）和知母（Rhizoma anemarrhenae）组成且具有免疫增强作用的复方制剂A、B、C对草鱼（Ctenopharyngodon idellus）组织形态和生长性能方面可能存在的风险，本试验将二龄草鱼随机分成12个试验组和2个对照组，分别连续投喂含有不同复方制剂的饲料和空白饲料，在试验第7、14、21、28天分别随机从对应试验组中采集5尾草鱼肝胰脏、脾脏、肾脏和肠道进行切片制作和组织形态分析；在试验第0、29天分别随机从对应试验组中采集10尾草鱼进行肥满度、增重率、特定生长率、脏体比和肠道承重力测定。结果表明，分别以2%、4%剂量添加于草鱼饲料中的复方制剂A、B、C对试验草鱼阶段增重率、特定生长率、饵料系数、肝胰体比、肾体比和肠道承重力系列指标均无异常影响。组织形态分析方面，复方制剂A对草鱼肝胰脏、脾脏、肾脏和肠道具有良好的安全性，而复方制剂B、C经连续投喂21天起，对应剂量组草鱼肝胰脏、肾脏逐渐呈现不同程度的组织病变。本文得出结论认为，即使完全符合允饲要求的天然植物品种，缺乏基于较全面的评价维度试验证明其具有使用安全性，则容易仅关注允饲天然植物在效果表达的表象，而忽略其诱发的组织病变造成组织器官的功能衰退。 |
| 关键词: 允饲植物 复方制剂 草鱼 组织形态 生长性能 安全性 |
| DOI：10.11693/hyhz20161200280 |
| 分类号:Q176;R285;S965.1 |
| 基金项目:广东省博士后科研基金专项资助，B150787号。 |
|
| THE EFFECT OF BOTANICAL PREPARATIONS ALLOWED FEEDING ON THE TISSUE MORPHOLOGY AND GROWTH PERFORMANCE OF GRASS CARP CTENOPHARYNGODON IDELLUS |
|
HUANG Jin-Lu1,2, LI Ai-Hua1, QIAN Xue-Qiao2, TAN Xiao-Chen2, WANG Zheng-Kai2
|
|
1.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;2.Animal Husbandry and Fisheries Research Center of Guangdong Haid Group Co., Limited, Guangzhou 511400, China
|
| Abstract: |
| Botanical preparation A, B and C have been reported to have immunostimulating effects in grass carp Ctenopharyngodon idellus. However, toxic response of grass carp to botanical preparation A, B and C has not been evaluated yet. The aim of the study was to analyze the effects of botanical preparation A, B and C on histological changes and growth performances in grass carp. Two-year-old grass carps were randomized into 14 groups; the 2 control groups were fed with commercial diet at a feeding rate of 3% body weight per day, while 12 experimental groups were fed with feed supplemented with 2% or 4% botanical preparation A, B, C at the same feeding rate. Tissues of hepatopancreas, spleen, kidney and intestine from the corresponding experimental groups were sampled on 7th, 14th, 21st and 28th day post feeding for histological analysis. And 10 grass carps of each group were dissected to estimate the condition factor, weight gain rate, specific growth rate, organ index and intestinal bearing capacity on the 0th and 29th post feeding. Results show that botanical preparation A, B, C had little effect on the condition factor, weight gain rate, specific growth rate, organ body ratio and intestinal bearing capacity of grass carp. On histology, tissues of grass carps fed with botanical preparation A showed no pathological changes, while the hepatopancreas and kidney of botanical preparation B and C exhibited different degrees of pathological lesions after 21 days feeding. In conclusion, botanical preparation A is safe to be used as an feed additive. Results suggest that safety evaluation of botanical preparations is required before use, even though they fully meet the requirements. |
| Key words: plant feed additives botanical preparation grass carp Ctenopharyngodon idellus tissue morphology growth performance safety |
