| 摘要: |
| 脂肪酸去饱和酶（FAD，fatty acyl desaturase）及延伸酶（ELOVL，elongases of very long chain fatty acids）在鱼类脂肪代谢过程中发挥了重要作用。利用RT-PCR克隆得到瓦氏黄颡鱼（Pelteobagrus vachellii）肝脏中控制高不饱和脂肪酸合成的（FAD2）和（ELOVL5）基因cDNA序列。瓦氏黄颡鱼FAD2 cDNA片段长2041bp，编码447个氨基酸，含有3个组氨酸簇（HDxGH，HxxHH，QxxHH），含亚铁血红素结合基序（HPGG）的类似细胞色素b5结构域等。瓦氏黄颡鱼ELOVL5 cDNA片段长1065bp，编码294个氨基酸，含有组氨酸簇（HxxHH）、内质网停留信号（K、R）和4个ELO共有的保守区域（KxxExxDT，QxxFLHxYHH，NxxxHxxMYxYY，TxxQxxQ）等结构域。荧光定量PCR分析表明，FAD2和ELOVL5 mRNA在瓦氏黄颡鱼脑、肝脏的表达量最高，显著高于肠道、脾脏、肾脏、鳃等组织。结果表明，瓦氏黄颡鱼具有合成高不饱和脂肪酸的关键酶FAD2和ELOVL5，且肝脏为合成高不饱和脂肪酸的主要场所。 |
| 关键词: 脂肪酸去饱和酶(FAD2) 脂肪酸延伸酶(ELOVL5) 瓦氏黄颡鱼 cDNA |
| DOI：10.11693/hyhz20170400088 |
| 分类号:Q789 |
| 基金项目:国家自然科学基金项目，31402305号；四川省科技厅应用基础项目，2017JY0161号；四川省“十三五”育种攻关项目，2016NYZ0024号。 |
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| MOLECULAR CLONING AND EXPRESSION OF FATTY ACID DESATURASE AND ELONGASE GENES IN DARKBARBEL CATFISH PELTEOBAGRUS VACHELLII |
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QIN Chuan-Jie1, WEN Zheng-Yong1, YUAN Deng-Yue1, SHAO Ting1, GONG Quan2
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1.Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang 641112, China;2.Fisheries Institute, Sichuan Academy of Agricultural Sciences, Chengdu 611731, China
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| Abstract: |
| Understanding the endogenous synthesis of long-chain polyunsaturated fatty acids is of benefit to fish feed formulation. A FADS2 and an ELOVL5 cDNA were cloned containing open reading frames (ORF) of 1338 base pair (bp) and 864 bp specifying proteins of 447 and 294 amino acids, respectively. The deduced amino acid sequence of Pelteobagrus vachellii FADS2 possessed conserved motif and included the histidine boxes, cytochrome b5 domain, transmembrane regions, and ELOVL 5 containing histidine boxes, endoplasmic reticulum retention signal domain, and transmembrane regions. In addition, FADS2 and ELOVL 5 mRNA distributed mainly in the brain and liver, and expressed significantly higher than that of intestine, spleen, kidney, gill, heart and muscle. These results indicate that P. vachellii possessed the desaturase and elongase, and the liver was the main tissues for synthesis of long-chain polyunsaturated fatty acids. |
| Key words: fatty acid desaturase fatty acid elongase Pelteobagrus vachellii cDNA |
