| 摘要: |
| 本文研究了不同浓度（0，0.1，1.0，4.0和8.0mg/L）的植物生长调节剂烯效唑处理20天内对铜藻（Sargassum horneri）的生长及抗氧化系统的影响。结果表明，烯效唑显著抑制了铜藻的生长，各处理组藻体的长度和比生长速率（SGR）均显著低于对照组，且烯效唑处理浓度越高，抑制作用越明显。0.1和1.0mg/L烯效唑处理组的叶绿素a、类胡萝卜素和可溶性蛋白的含量均显著提高，可溶性糖的含量在前5天内有短暂的促进，而4.0和8.0mg/L处理组的叶绿素a、类胡萝卜素和可溶性蛋白的含量显著下降，而可溶性糖的含量显著升高。抗氧化防御指标的变化也呈现差异性，各处理组的超氧化物歧化酶（SOD）活性在5d时均显著提高，而随着处理时间的延长，低浓度（≤ 1.0mg/L）处理组SOD活性恢复到对照组水平，高浓度（≥ 4.0mg/L）处理组SOD活性仍保持在较高水平，显著高于对照组。0.1mg/L处理组的过氧化氢酶（CAT）活性在实验10d时才显著升高，表现为逐渐诱导的现象，丙二醛（MDA）含量在20d时略低于对照组；而其他三个高浓度处理组的CAT活性在实验期间，均显著升高，MDA含量20d时也显著高于对照。以上研究结果丰富了植物生长调节剂对藻类生长影响的研究体系，为烯效唑在大型藻类中的合理应用提供了理论依据。 |
| 关键词: 铜藻 烯效唑 生长 抗氧化系统 |
| DOI：10.11693/hyhz20170500137 |
| 分类号:S917.3 |
| 基金项目:海洋公益性行业科研专项经费项目，201505022号，201405040号；山东省2017年度农业重大应用技术创新项目。 |
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| EFFECTS OF UNICONAZOLE ON GROWTH AND ACTIVITY OFANTIOXIDATION SYSTEM IN SARGASSUM HORNERI |
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LÜ Fang1,2, DIND Gang1,2, WU Hai-Yi1,2, XIN Mei-Li1,2, GUO Wen1
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1.Marine Biology Institute of Shandong Province, Qingdao 266104, China;2.Qingdao Macroalgae Engineering Technology Research Center, Qingdao 266104, China
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| Abstract: |
| The effects of uniconazole, a plant growth regulator, in different concentrations (0, 0.1, 4.0, and 8.0mg/L) on growth and antioxidant system of algae Sargassum horneri were studied in a 20-day experiment. The results show that uniconazole inhibited significantly the algae growth, size, and specific growth rate (SGR) in all concentration treatment groups were significantly lower than those of the control, and the higher uniconazole concentration, the greater inhibition. The contents of chlorophyll a, carotenoid, and soluble protein in 0.1 and 1.0mg/L treatment groups significantly increased, and soluble sugar content showed a temporary promotion in the first 5 days. On the other hand, chlorophyll a, carotenoid, and soluble protein contents in higher concentration (≥ 4.0mg/L) groups decreased obviously, and the soluble sugar content increased significantly. The antioxidant index showed different variation. The superoxide dismutase (SOD) activities of all treatment group showed an increasing trend in the first 5d and then the low concentration (≤ 1.0mg/L) groups returned to control level, while high concentration (≥ 4.0mg/L) groups remained at a high level, significantly higher than the control. Catalase (CAT) activity of 0.1mg/L group increased on Day 10, showing gradual induction, while the CAT activities in other three groups increased significantly. Malondialdehyde (MDA) content in 0.1mg/L group was lower than the control on Day 20, while in high concentration (≥ 1.0mg/L) groups it increased significantly. These findings may enrich understanding of the impacts of plant growth regulators on algae growth with a theoretical tool for uniconazole application on macroalgae. |
| Key words: Sargassum horneri uniconazole growth antioxidant system |
