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| 基于综合生物标志物响应评价苯并芘对脊尾白虾(Exopalaemon carinicauda)的毒性效应 |
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李磊1,2, 刘金殿1, 黄士林3, 唐峰华2, 陈玉生4, 赵永超4, 许呈林4, 王云龙2, 沈新强2, 蒋玫2
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1.农业部淡水渔业健康养殖重点实验室 浙江省鱼类健康与营养重点实验室 浙江省淡水水产研究所 湖州 313001;2.中国水产科学研究院东海水产研究所 上海 200090;3.海盐县海洋与渔政管理站 嘉兴 314300;4.江苏省海洋水产研究所 南通 226007
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| 摘要: |
| 苯并[a]芘（BaP）是一类典型多环芳烃（PAHs）污染物。为了定量评价BaP对脊尾白虾（Exopalaemon carinicauda）的毒性效应，采用水体暴露实验，研究了脊尾白虾肝胰脏、肌肉组织的5种生物标志物（EROD、GST、SOD、CAT、MDA）对BaP暴露的响应，并应用综合生物标志物响应（IBR）定量评价了BaP对肝胰脏、肌肉组织的毒性差异。结果显示：肝胰脏、肌肉组织的EROD、GST、SOD、CAT活性整体呈现诱导-抑制的规律性变化，除了0.05μg/L实验组CAT外，其余各实验组肝胰脏组织生物标志物活性在实验结束时均显著高于对照组（P<0.05），除了0.45μg/L实验组EROD、CAT外，其余各浓度组肌肉组织生物标志物活性在实验结束时均与对照组无显著性差异（P>0.05），MDA含量呈现持续上升的趋势，在实验结束时各实验组均显著高于对照组（P<0.05）；IBR值存在时间效应和剂量效应。研究结果表明，肝胰脏、肌肉组织对BaP的暴露响应存在差异性，肝胰脏生物标志物综合应激活性高于肌肉组织。 |
| 关键词: 苯并芘 脊尾白虾 综合生物标志物响应 |
| DOI：10.11693/hyhz20170800218 |
| 分类号:X171 |
| 基金项目:农业部淡水渔业健康养殖重点实验室开放课题，ZJK201509号；上海市科学技术委员会项目，12231203403号；中国水产科学研究院基本科研业务费项目，2014A02XK01号。 |
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| INTEGRATED ASSESSMENT OF BIOMARKER RESPONSES IN EXOPALAEMON CARINICAUDA EXPOSED TO BENZO[A]PYRENE |
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LI Lei1,2, LIU Jin-Dian1, HUANG Shi-Lin3, TANG Feng-Hua2, CHEN Yu-Sheng4, ZHAO Yong-Chao4, XU Cheng-Lin4, WANG Yun-Long2, SHEN Xin-Qiang2, JIANG Mei2
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1.Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China;2.East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;3.Ocean and Fishery Management Station of Haiyan County, Jiaxing 314300, China;4.Marine Fisheries Research Institute of Jiangsu, Nantong 226007, China
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| Abstract: |
| Benzo[a]pyrene (BaP) is a representative polycyclic aromatic hydrocarbon (PAHs), which is ubiquitous in the environment. To determine the toxic effects induced by BaP, biomarkers (EROD, GST, SOD, CAT, MDA) responses in muscle and hepatopancreas of Exopalaemon carinicauda exposed to BaP were studied, and the toxicity of BaP assessed by integrated biomarker response (IBR). The results show that these biomarkers showed certain induction-inhibition. The activities in all exposure concentrations in hepatopancreas were higher than that of the control (P<0.05) except for CAT in 0.05μg/L, and could resume to a normal level (P>0.05) except for EROD and CAT in 0.45μg/L. Meanwhile, the content of MDA in hepatopancreas presented a rising trend and higher than the control (P<0.05). The IBR (integrated biomarker response) was proved as a useful index to the quantification of various biomarker responses to toxic chemical exposures, and showed that the sensitivity of hepatopancreas was higher than that of the muscle. |
| Key words: benzo[a]pyrene Exopalaemon carinicauda integrated biomarker response |