| 摘要: |
| 粉叶马尾藻（Sargassum glaucescens）是台湾地区潮间带的重要大型藻类，其对紫外线增强的生理响应特征尚不清楚。本研究关注粉叶马尾藻在急性紫外线辐射（Ultraviolet radiation B，UVB）下的生理指标变化，探索了粉叶马尾藻在急性UVB辐照下抗氧化系统的瞬时反应过程。结果表明：低强度组[0.5W/（m2·s）]藻体在24h内未出现显著氧化损伤和蛋白质含量变化，但是细胞活力在辐照12h后显著降低，藻体超氧化物歧化酶（SOD）酶活性随辐照时间延长而降低，过氧化氢酶（CAT）酶活性呈现升高后下降的趋势，抗坏血酸过氧化物酶（APX）酶活性没有出现显著降低；高强度组[2W/（m2·s）]藻体在辐照3h后藻体出现明显氧化损伤，6h后细胞活力和蛋白质含量显著降低，藻体SOD酶活性随辐照时间延长而降低，CAT和APX酶活性呈现升高后降低的趋势，辐射12h后即显著降低。低强度UVB辐射对粉叶马尾藻的影响相对较小，表明粉叶马尾藻对UVB辐射具有一定耐受性；而高强度UVB辐射的影响较为显著，意味着UVB辐射的持续增强将超出粉叶马尾藻的耐受限度，进而造成不利影响。本研究结果初步揭示了粉叶马尾藻对于急性UVB辐射的增强的生理响应特征，为进一步研究UVB辐射增强的生态学效应提供参考依据。 |
| 关键词: 紫外线辐射(Ultraviolet radiation B,UVB) 粉叶马尾藻 抗氧化系统 |
| DOI：10.11693/hyhz20180200031 |
| 分类号:X173 |
| 基金项目:国家自然科学基金项目，41476091号。 |
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| EFFECT OF ACUTE UVB RADIATION ON THE ANTIOXIDANT SYSTEM IN SARGASSUM GLAUCESCENS |
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YUAN Shi-Peng1,2, TANG Xue-Xi1,3, YU Qi1, ZANG Yu1, LEE T M4, ZHOU Bin1,3
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1.Ocean University of China, Qingdao 266000, China;2.Qingdao National Laboratory for Marine Science and Technology Joint Laboratory for Deep Blue Fishery Engineering, Qingdao 266000, China;3.Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China;4.National Sun Yat-sen University, Gaoxiong 000800, China
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| Abstract: |
| Sargassum glaucescens is an important macroalga in Taiwan, South China. However, its physiological response to ultraviolet radiation remains unclear. This study focuses on the physiological response of S. glaucescens under acute ultraviolet radiation B (UVB). A low-intensity UVB[0.5W/(m2·s)] and a high-intensity UVB[2W/(m2·s)] irradiation group was set up. The results show that the low-intensity group showed no significant oxidative damage and protein content variation in 24h; however, the cell viability was reduced significantly in 12h. SOD activity decreased with the irradiation time, CAT activity increased and then decreased, APX activity did not decrease significantly. In the high-intensity group, an obvious oxidative damage occurred in 3h. The cell activity and protein content decreased significantly in 6h. SOD activity decreased with the irradiation time. Both CAT and APX activities increased at first and then decreased, and enzyme activity decreased significantly in 12h. Therefore, the low-intensity radiation effect on S. glaucescens was relatively small as S. glaucescens could tolerate UVB radiation to some degrees. However, the effect of high-intensity UVB radiation was significant. A continuous increase of high-intensity UVB radiation would exceed the tolerance limit of S. glaucescens, and then lead to an adverse effect. This study revealed preliminarily the physiological response of S. glaucescens to an increasing acute UVB radiation, providing a reference to study the ecological effect of UVB radiation enhancement. |
| Key words: ultraviolet radiation B (UVB) Sargassum glaucescens antioxidant system |
